Tosun Murat, Tosun Emine, Kalkan Serpil, Avunduk Mustafa Cihat
Medical Faculty, Department of Histology and Embryology, University of Kocatepe, Afyonkarahisar 03200, Turkey.
J Mol Histol. 2007 Aug;38(4):271-4. doi: 10.1007/s10735-007-9097-2. Epub 2007 May 22.
P53 is a tumor suppressor gene and a critical component of cellular mechanisms that respond to genotoxic stresses. During normal fetal development, some of these cells lose their genomic stability because of intensive cell proliferation. They arrest cell cycle progression and repair genomic stability by p53 induction or die via apoptosis. If p53 is overexpressed, some structures may have different abnormalities. This study was conducted to investigate normal p53 expression in human male gonads during second trimester. Twenty one normal human male fetuses' testes in 2nd trimester were processed and immunohistochemistry was applied. The spermatogonia with nuclear and perinuclear staining, were accepted as p53 (+). The number of p53 (+) spermatogonia was counted in randomly 10 different seminiferous tubules. The results suggest that p53 expression in gonads of human male fetuses significantly increases in the 20th week.
P53是一种肿瘤抑制基因，也是细胞对基因毒性应激反应机制的关键组成部分。在正常胎儿发育过程中，由于细胞的大量增殖，其中一些细胞会失去基因组稳定性。它们会通过诱导p53来阻止细胞周期进程并修复基因组稳定性，或者通过凋亡死亡。如果p53过度表达，某些结构可能会出现不同的异常情况。本研究旨在调查妊娠中期人类男性性腺中p53的正常表达情况。对21例妊娠中期正常男性胎儿的睾丸进行处理，并应用免疫组织化学方法。细胞核和核周染色的精原细胞被视为p53(+)。在随机选取的10个不同生精小管中计数p53(+)精原细胞的数量。结果表明，人类男性胎儿性腺中p53的表达在第20周时显著增加。
