Yathavakilla Santha Ketavarapu V, Caruso Joseph A
Department of Chemistry, University of Cincinnati/Agilent Technologies, Metallomics Center of the Americas, P.O. Box 210172, Cincinnati, OH 45221, USA.
Anal Bioanal Chem. 2007 Oct;389(3):715-23. doi: 10.1007/s00216-007-1458-x. Epub 2007 Jul 26.
An attempt was made to study selenium (Se) and mercury (Hg) interactions in plants, specifically soybean (Glycine max), by inductively coupled plasma mass spectrometric detection. Greenhouse-cultivated plants were subjected to treatment with different regimens of Se and Hg and analyzed for their metabolized species in roots, stems, leaves, pods and beans. Most of the water-soluble Hg was found to be localized in the roots in association with Se in a high molecular weight entity, as identified by size exclusion chromatography. This entity was also extracted in protein specific isolate, but it resisted enzymatic breakdown. Complete breakdown of this high molecular weight species was accomplished by acid hydrolysis. Optimization of the conditions for acid hydrolysis is discussed. Hg and Se species found in root extract were studied by ion-pairing chromatography. In a sub-study, the Se distribution pattern was found to be unaffected by the presence of Hg, but the amount of Se assimilated was found to be higher in plants coexposed to Hg.
通过电感耦合等离子体质谱检测，对植物，特别是大豆（Glycine max）中硒（Se）和汞（Hg）的相互作用进行了研究。对温室栽培的植物进行不同硒和汞处理方案的处理，并分析其根、茎、叶、豆荚和豆子中的代谢产物。通过尺寸排阻色谱法鉴定，发现大部分水溶性汞与硒结合存在于根中，形成高分子量物质。该物质也可从蛋白质特异性分离物中提取，但能抵抗酶解。通过酸水解可实现这种高分子量物质的完全分解。讨论了酸水解条件的优化。通过离子对色谱法研究了根提取物中汞和硒的形态。在一项子研究中，发现硒的分布模式不受汞存在的影响，但在同时暴露于汞的植物中，硒的同化量更高。
