Dodge G R, Kovalszky I, McBride O W, Yi H F, Chu M L, Saitta B, Stokes D G, Iozzo R V
Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Genomics. 1991 Sep;11(1):174-8. doi: 10.1016/0888-7543(91)90115-u.
The nucleotide sequence of a 916-bp human cDNA clone isolated from a human colon lambda gt11 cDNA library was determined. Sequence analysis showed this cDNA to have 88% homology to the nucleotide sequence of the heavy chain of rat clathrin. The deduced amino acid sequence was 98.7% identical to the rat sequence, a change of only four amino acids. The mRNA identified in both human and rat cells with the human clathrin clone revealed transcripts of approximately 6.5 kb, which is consistent with the predicted 180 kDa molecular weight of the clathrin heavy chain. Southern analysis of human/rodent somatic cell hybrids localized the human clathrin heavy chain gene (CLTC) to chromosome 17. Additional analyses using panels of human/rodent somatic cell hybrids with specific chromosomal translocations and deletions mapped the human clathrin heavy chain gene locus to 17q11-qter.
从人结肠λgt11 cDNA文库中分离出一个916 bp的人cDNA克隆，并测定了其核苷酸序列。序列分析表明，该cDNA与大鼠网格蛋白重链的核苷酸序列有88%的同源性。推导的氨基酸序列与大鼠序列的同一性为98.7%，仅四个氨基酸发生了变化。用人网格蛋白克隆在人和大鼠细胞中鉴定出的mRNA显示约6.5 kb的转录本，这与预测的180 kDa网格蛋白重链分子量一致。对人/啮齿动物体细胞杂种的Southern分析将人网格蛋白重链基因（CLTC）定位到17号染色体。使用具有特定染色体易位和缺失的人/啮齿动物体细胞杂种面板进行的其他分析将人网格蛋白重链基因座定位到17q11 - qter。
