Aref Salah, Salama O, Shamaa S, El-Refaie M, Mourkos H
Hematology Unit, Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura, Egypt.
Hematology. 2007 Aug;12(4):319-24. doi: 10.1080/10245330701340759.
Angiogenesis is an important event in the survival and progression of solid tumors. The angiogenic status and the exact role of the angiogenic cytokines in lymphoid leukemia has not been fully elucidated. We have investigated the profile of the systemic components of angiogenic regulation in B-lineage acute lymphoblastic leukemia (B-ALL) and B-chronic lymphocytic leukemia (B-CLL), namely vascular endothelial growth factor (VEGF), tumor necrosis factor-alpha (TNF-alpha), endostatin and matrix metalloproteinase-9 (MMP-9) using enzyme-linked immunosorbent assay (ELISA). In B-ALL patients, sVEGF, and MMP-9 were significantly lower than control levels at diagnosis (p < 0.001) and increased to near control levels in remission (p>0.05). Both serum TNF-alpha and endostatin levels showed no significant difference at diagnosis (p>0.05) and in remission (p>0.05) compared to control levels. VEGF, TNF-alpha, MMP-9 and endostatin levels were not significantly correlated with peripheral white cell count or bone marrow blast cell count, but were positively correlated with platelet count. In B-CLL patients, serum VEGF, MMP-9 and TNF-alpha were significantly higher (p < 0.001 = 0.009, 0.007, respectively) and decreased to near control levels in remission (p>0.05 for all). Serum endostatin levels showed no significant difference at diagnosis and in remission compared to control levels (p>0.05). A significant positive correlation between VEGF, TNF-alpha, MMP-9 and peripheral white cell counts, bone marrow lymphocytic count and platelets count were found. In conclusion, our data suggest that the driving forces of angiogenic factors (VEGF, TNF-alpha and MMP-9) in adult B-ALL appears different from that in B-CLL patients.
血管生成是实体瘤存活和进展过程中的一个重要事件。血管生成状态以及血管生成细胞因子在淋巴细胞白血病中的确切作用尚未完全阐明。我们使用酶联免疫吸附测定（ELISA）研究了B系急性淋巴细胞白血病（B-ALL）和B慢性淋巴细胞白血病（B-CLL）中血管生成调节的全身成分概况，即血管内皮生长因子（VEGF）、肿瘤坏死因子-α（TNF-α）、内皮抑素和基质金属蛋白酶-9（MMP-9）。在B-ALL患者中，可溶性VEGF（sVEGF）和MMP-9在诊断时显著低于对照水平（p<0.001），缓解期升高至接近对照水平（p>0.05）。血清TNF-α和内皮抑素水平在诊断时（p>0.05）和缓解期（p>0.05）与对照水平相比均无显著差异。VEGF、TNF-α、MMP-9和内皮抑素水平与外周白细胞计数或骨髓原始细胞计数无显著相关性，但与血小板计数呈正相关。在B-CLL患者中，血清VEGF、MMP-9和TNF-α显著更高（分别为p<0.001 = 0.009、0.007），缓解期降至接近对照水平（均为p>0.05）。血清内皮抑素水平在诊断时和缓解期与对照水平相比无显著差异（p>0.05）。发现VEGF、TNF-α、MMP-9与外周白细胞计数、骨髓淋巴细胞计数和血小板计数之间存在显著正相关。总之，我们的数据表明，成人B-ALL中血管生成因子（VEGF、TNF-α和MMP-9）的驱动因素似乎与B-CLL患者不同。
