Mizuno Yuji, Guyon Jeffrey R, Okamoto Koichi, Kunkel Louis M
Department of Neurology, Gunma University Graduate School of Medicine, 3-39-22 Showa, Maebashi, Gunma, Japan.
Muscle Nerve. 2007 Oct;36(4):497-504. doi: 10.1002/mus.20847.
beta-Synemin has been identified as an alpha-dystrobrevin-interacting protein in human muscle, although at least two synemin transcripts are expressed in brain. To understand synemin's function in neural tissue, in situ and immunohistochemical analyses were performed to identify where alpha- and beta-synemin are expressed in the brain of C57BL/6 and mdx (dystrophin null) mice. This analysis shows that the alpha- and beta-synemin transcripts and their encoded proteins colocalize in neurons, especially in the midbrain and pons. Since alpha-dystrobrevin-1 and synemin do not colocalize in brain as in muscle, this suggests that another member of the dystrophin-associated protein complex might interact with synemin in brain. In support of this, synemin mRNA expression was decreased in mdx mice, suggesting that synemin transcription is linked to dystrophin expression. Our findings show where synemin is expressed in brain and allow one to speculate with regard to its function in neural tissue.
β-联丝蛋白已被确定为人类肌肉中与α-肌营养不良蛋白结合的蛋白，尽管在大脑中至少表达两种联丝蛋白转录本。为了了解联丝蛋白在神经组织中的功能，进行了原位和免疫组织化学分析，以确定α-和β-联丝蛋白在C57BL/6和mdx（肌营养不良蛋白缺失）小鼠大脑中的表达位置。该分析表明，α-和β-联丝蛋白转录本及其编码蛋白在神经元中共定位，尤其是在中脑和脑桥。由于α-肌营养不良蛋白-1和联丝蛋白在大脑中不像在肌肉中那样共定位，这表明肌营养不良蛋白相关蛋白复合物的另一个成员可能在大脑中与联丝蛋白相互作用。支持这一观点的是，mdx小鼠中联丝蛋白mRNA表达降低，表明联丝蛋白转录与肌营养不良蛋白表达有关。我们的研究结果显示了联丝蛋白在大脑中的表达位置，并让人能够推测其在神经组织中的功能。
