[人血清中原卟啉IX的自体荧光光谱研究]

[Study of autofluorescence spectra of protoporphyrin IX in human serum].

作者信息

Guo Xing-jia, Li Xiao-zhou, Xu Shu-kun, Tong Jian, Jiang Yu-chun, Kang Ping-li

机构信息

Chemistry Department of Northeast University, Shenyang 110004, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2007 May;27(5):995-8.

PMID:17655122

Abstract

The fluorescence spectra of protoporphyrin IX (PPIX) solutions and human serum samples were measured and analyzed under physiological conditions. The experimental results showed that the fluorescence of PPIX in human serum was mainly derived from PPIX-serum albumin complex. Moreover, the effects of serum albumin and PPIX on the PPIX emission fluorescence were also investigated. Compared with pure PPIX solution, not only a red-shift of PPIX fluorescence peak was found for PPIX+albumin solution, but also albumin had a fluorescence enhancement effect on protoporphyrin IX. For the mixture of PPIX+albumin, with the increase of PPIX the wavelength of the PPIX emission peak will increased a little when its concentration was less than 0.8 x 10(-5) mol x L(-1), but was nearly invariable when its concentration was more than 0.8 x 10(-5) mol x L(-1).

摘要

在生理条件下测量并分析了原卟啉IX（PPIX）溶液和人血清样品的荧光光谱。实验结果表明，人血清中PPIX的荧光主要来源于PPIX-血清白蛋白复合物。此外，还研究了血清白蛋白和PPIX对PPIX发射荧光的影响。与纯PPIX溶液相比，PPIX+白蛋白溶液不仅出现PPIX荧光峰红移，而且白蛋白对原卟啉IX有荧光增强作用。对于PPIX+白蛋白混合物，当PPIX浓度小于0.8×10⁻⁵mol·L⁻¹时，随着PPIX浓度增加，PPIX发射峰波长略有增加，但当浓度大于0.8×10⁻⁵mol·L⁻¹时，波长几乎不变。