Department of General Surgery, Regional Specialist Hospital, Czestochowa, Poland.
Department of General Surgery, Jagiellonian University Medical College; Cracow, Poland.
J Physiol Pharmacol. 2023 Aug;74(4). doi: 10.26402/jpp.2023.4.10. Epub 2023 Oct 16.
Photodynamic therapy is a high-target, low-invasive treatment utilized to manage a variety of malignant diseases and precancerous lesions. Protoporphyrin IX (PpIX) is one of the most important photosensitizers used in photodynamic therapy, carried to the cancer tissue by serum albumin. Its delivery by transport protein is one of the major factors in determining the efficacy of photodynamic therapy. The distribution of the albumin-PpIX complexes to the target tissue enables the accomplishment of an optimal PDT effect. This study aimed to assess in vitro the stability of spectrofluorimetric spectra of albumin-PpIX complexes. The experiment used three chemicals: PpIX, human serum albumin (HSA), and bovine serum albumin (BSA). Spectral data was recorded using a Kontron SFM-25 Instrument AG, at two excitation wavelengths λ=280 nm and 295 nm. A concentration of 1x10M of PpIX, in combination with 1.25x10M of HSA and 4x10M of BSA, have been recorded repetitively for ten days and compared to the initial spectrum. The maximum of PpIX fluorescence changed significantly on the first day following sample preparation. The maximum of PpIX - serum albumin complex was stable 10 and 4 days for HSA and 5 and 2 days for BSA for λ=280 nm and 295 nm, respectively. The formation of a complex between PpIX and serum albumin was seen to extend the stability of the spectrofluorimetric spectrum. However, a less significant effect was observed in the case of BSA, which could most plausibly be attributed to the variations in primary structure between HSA and BSA, leading to discernible variations in spectroscopic measurements.
光动力疗法是一种高靶向、低侵袭性的治疗方法，用于治疗多种恶性疾病和癌前病变。原卟啉 IX（PpIX）是光动力疗法中最重要的光敏剂之一，由血清白蛋白携带到癌症组织中。其通过转运蛋白的输送是决定光动力疗法疗效的主要因素之一。白蛋白-PpIX 复合物向靶组织的分布使光动力疗法达到最佳效果成为可能。本研究旨在评估白蛋白-PpIX 复合物的光谱荧光稳定性。实验使用了三种化学物质：PpIX、人血清白蛋白（HSA）和牛血清白蛋白（BSA）。使用 Kontron SFM-25 仪器 AG 记录光谱数据，激发波长λ=280nm 和 295nm。记录了浓度为 1x10M 的 PpIX 与 1.25x10M 的 HSA 和 4x10M 的 BSA 结合后连续十天的光谱数据，并与初始光谱进行了比较。样品制备后第一天，PpIX 荧光的最大值发生了显著变化。对于λ=280nm 和 295nm，HSA 和 BSA 中的 PpIX- 血清白蛋白复合物的最大值分别稳定了 10 天和 4 天以及 5 天和 2 天。PpIX 与血清白蛋白形成复合物延长了光谱荧光的稳定性。然而，在 BSA 的情况下观察到的效果不太显著，这最有可能归因于 HSA 和 BSA 之间一级结构的差异，导致光谱测量存在明显差异。
