Mónaco C I, Rollán M C, Nico A I
Laboratorio de Fitopatología. Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata (UNLP) & Comisión de Investigaciones Científicas de La Provincia de Buenos Aires, Buenos Aires, Argentina.
Rev Iberoam Micol. 1998 Jun;15(2):81-4.
The ability to parasitise Sclerotinia sclerotiorum and the effect on apothecia production was evaluated for the following antagonists: Trichoderma harzianum; Trichoderma koningii; Gliocladium roseum and Chaetomium globosum. Plastic trays were filled with of steam-sterilized soil. Each one of them was infested with sclerotia of S. sclerotiorum and the culture of the antagonists. The trays were kept in a greenhouse and after 30, 60 and 90 days, evaluations were made. The rates of carpogenic germination, myceliogenic germination, mycoparasitism and destruction were evaluated. To assess carpogenic germination, the sclerotia were put in a growth chamber over moistened filter paper at 20 -/+ 2 degrees C and 12 light hours. The rates of myceliogenic germination and mycoparasitism were evaluated on Petri dishes with 2% APD. Antagonists effect on carpogenic germination was observed one month after the start of the assay. In the evaluation made at 60 and 90 days, T. harzianum; T. koningii and G. roseum kept inhibitory properties. Such inhibition was not observed in the trays containing C. globosum. In the evaluations made at 30 days, mycoparasitism rate was high in the trays with T. harzianum; T. koningii and G. roseum. G. roseum and T. harzianum destroy S. sclerotiorum sclerotia.
哈茨木霉、康宁木霉、粉红粘帚霉和球毛壳菌。塑料托盘装满经蒸汽灭菌的土壤。每个托盘接种核盘菌菌核和拮抗菌培养物。托盘置于温室中，30天、60天和90天后进行评估。评估了产孢萌发率、菌丝体萌发率、重寄生作用和破坏情况。为评估产孢萌发，将菌核置于生长室中，在20±2℃、光照12小时的条件下，放在湿润的滤纸上。在含有2%酸性蛋白胨的培养皿上评估菌丝体萌发率和重寄生作用。在试验开始一个月后观察拮抗菌对产孢萌发的影响。在60天和90天的评估中，哈茨木霉、康宁木霉和粉红粘帚霉保持抑制特性。在含有球毛壳菌的托盘中未观察到这种抑制作用。在30天的评估中，含有哈茨木霉、康宁木霉和粉红粘帚霉的托盘中重寄生率较高。粉红粘帚霉和哈茨木霉可破坏核盘菌菌核。
