Izumi Toshio, Eida Tarou, Matsumoto Noriyoshi, Inoue Hiroshi
Department of Operative Dentistry and Endodontology, Fukuoka Dental College, Fukuoka, Japan.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Oct;104(4):e133-7. doi: 10.1016/j.tripleo.2007.04.023. Epub 2007 Jul 26.
Metallothionein (MT) is an intracellular cysteine-rich protein associated with cell proliferation and differentiation. Our objective was to examine immunohistochemically the localization of MT in the rat dental pulp after cavity preparation.
Cavities were prepared on the upper first molars of 9 rats. Specimens were collected at 1, 3, and 5 days after cavity preparation, and paraffin sections were made. For double-immunohistochemical staining, anti-MT monoclonal antibody (E9) and anti-proliferating cell nuclear antigen (PCNA) monoclonal antibody (PC10) were applied.
At 3 days after cavity preparation, some odontoblasts corresponding to the cavity, many pulp cells, and some endothelial cells in the pulp under the cavity showed both MT- and PCNA-positive immunostainings.
Metallothionein was detected in the dental pulp after pulp injury, and it is likely that MT is closely related to the proliferation of newly differentiating odontoblasts and angiogenesis during the healing process.
金属硫蛋白（MT）是一种与细胞增殖和分化相关的富含半胱氨酸的细胞内蛋白。我们的目的是通过免疫组织化学方法检测大鼠牙髓在制备洞型后MT的定位。
在9只大鼠的上颌第一磨牙制备洞型。在制备洞型后1、3和5天收集标本，制作石蜡切片。进行双重免疫组织化学染色时，应用抗MT单克隆抗体（E9）和抗增殖细胞核抗原（PCNA）单克隆抗体（PC10）。
在制备洞型后3天，与洞型对应的一些成牙本质细胞、许多牙髓细胞以及洞型下方牙髓中的一些内皮细胞显示MT和PCNA阳性免疫染色。
牙髓损伤后在牙髓中检测到金属硫蛋白，MT很可能在愈合过程中与新分化的成牙本质细胞的增殖和血管生成密切相关。
