用于透射电子显微镜的染色后网格：传统方法与替代方案

Poststaining grids for transmission electron microscopy: conventional and alternative protocols.

作者信息

Ellis E Ann

机构信息

Microscopy and Imaging Center, Texas A&M University, College Station, TX, USA.

出版信息

Methods Mol Biol. 2007;369:97-106. doi: 10.1007/978-1-59745-294-6_6.

DOI:10.1007/978-1-59745-294-6_6

PMID:17656748

Abstract

Poststaining ultrathin sections on grids is an essential process in preparing specimens for examination using transmission electron microscopy. This process commonly consists of staining in aqueous solutions of uranyl acetate followed by lead citrate and can result in good consistent quality staining if certain precautions are taken. This chapter covers conventional and alternative methods for poststaining ultrathin sections, including microwave-assisted procedures and corrective measures when problems are encountered.

摘要

在载网上对超薄切片进行复染是使用透射电子显微镜制备用于检查的标本的一个重要过程。这个过程通常包括在醋酸铀水溶液中染色，然后用柠檬酸铅染色，如果采取某些预防措施，可得到质量一致良好的染色效果。本章涵盖了对超薄切片进行复染的传统方法和替代方法，包括微波辅助程序以及遇到问题时的纠正措施。