el Okbi L M, Morsy T A, el-Shayeb F A, Salama M M, Abou Gamrah M M
Department of Parasitology, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, Egypt.
J Egypt Soc Parasitol. 1991 Dec;21(3):641-55.
Fleas are widely distributed and partially host specific ectoparasites of man and animal. The aim of this work was to study the role of fleas as one of the causative allergens of bronchial asthma in Egyptian patients. Two flea extract antigens were locally prepared. The first from the head and the salivary glands and the second from the abdomen. The sensitivity of the first antigen (head and salivary glands) was evaluated among a known group of flea bite allergic individuals and normal control group using two dilutions 1/50 and 1/100, and proved to be sensitive. Regarding bronchial asthma and fleas, two groups of individuals were examined, asthmatic patients and control ones, using flea extract antigen and common inhalant antigens as skin test as well as precipitin test using flea extract antigen. This test was done by prick method using the routine allergens (house dust, cotton dust, mixed mould, mixed pollens, cat hair, dog hair, wool, feather) and also with locally prepared two flea extracts: one was prepared from the head and salivary glands, the second was prepared from the abdomen. Out of these sixty asthmatic patients, only six (10%) gave positive skin reactions to the antigens of fleas extract. All the control gave negative skin reaction. The precipitin test using the double immuno diffusion method gave positive results with one case who showed reaction to flea extract skin antigen.
跳蚤是广泛分布且部分具有宿主特异性的人和动物体表寄生虫。这项工作的目的是研究跳蚤作为埃及患者支气管哮喘致病变应原之一的作用。当地制备了两种跳蚤提取物抗原。第一种来自头部和唾液腺，第二种来自腹部。使用1/50和1/100两种稀释度，在一组已知的跳蚤叮咬过敏个体和正常对照组中评估了第一种抗原（头部和唾液腺）的敏感性，结果证明其具有敏感性。对于支气管哮喘和跳蚤，使用跳蚤提取物抗原和常见吸入性抗原进行皮肤试验以及使用跳蚤提取物抗原进行沉淀素试验，对两组个体进行了检查，即哮喘患者和对照组。该试验通过点刺法进行，使用常规变应原（屋尘、棉尘、混合霉菌、混合花粉、猫毛、狗毛、羊毛、羽毛）以及当地制备的两种跳蚤提取物：一种由头部和唾液腺制备，另一种由腹部制备。在这60名哮喘患者中，只有6名（10%）对跳蚤提取物抗原产生阳性皮肤反应。所有对照组均产生阴性皮肤反应。使用双向免疫扩散法进行的沉淀素试验在1例对跳蚤提取物皮肤抗原呈反应的病例中得到了阳性结果。
