Ornelles David A, Broughton-Shepard Robin N, Goodrum Felicia D
Methods Mol Med. 2007;131:83-101. doi: 10.1007/978-1-59745-277-9_7.
Adenoviruses (Ads) are small DNA tumor viruses that have played a pivotal role in understanding eukaryotic cell biology and viral oncogenesis. Among other cellular pathways, Ad usurps cell cycle progression following infection. Likewise, progression of the viral infection is influenced by the host cell cycle. We describe here methods developed for synchronizing dividing cell populations and for analysis of cell cycle synchrony by flow cytometry. Furthermore, three methods used to evaluate the outcome of Ad infection in synchronized cell populations are described. These include two assays for infectious centers and an assay for analyzing production of progeny virus by transmission electron microscopy. These methods have been used to demonstrate that Ads that fail to direct synthesis of the E1B 55-kDa or E4orf6 proteins replicate most effectively upon infecting cells in S phase.
腺病毒（Ads）是小型DNA肿瘤病毒，在理解真核细胞生物学和病毒致癌作用方面发挥了关键作用。在其他细胞途径中，腺病毒感染后会篡夺细胞周期进程。同样，病毒感染的进程也受宿主细胞周期的影响。我们在此描述了用于同步分裂细胞群体以及通过流式细胞术分析细胞周期同步性的方法。此外，还描述了用于评估腺病毒在同步化细胞群体中感染结果的三种方法。其中包括两种用于检测感染中心的试验以及一种通过透射电子显微镜分析子代病毒产生情况的试验。这些方法已被用于证明，无法指导合成E1B 55 kDa或E4orf6蛋白的腺病毒在感染处于S期的细胞时复制最为有效。
