Kashevarova A A, Sukhanova N N, Tolmacheva E N, Sazhenova E A, Lebedev I N
Tsitologiia. 2007;49(4):322-8.
The ploidy level of noncultivated extraembryonic tissues was studied by fluorescence in situ hybridization in 30 human I trimester spontaneous abortions with tetraploid or diploid-tetraploid karyotype after conventional cytogenetic analysis. Only thirteen embryos (43 %) were verified to be tetraploid that provides evidence for the hypothesis of placental cell polyploidization during long-term in vitro cultivation. It is shown that preferred compartmentalization of tetraploid cells in the inner cell mass derivatives is associated with blighted ovum - the most severe type of human embryo dysmorphogenesis.
通过荧光原位杂交技术，对30例孕早期自然流产且经传统细胞遗传学分析显示为四倍体或二倍体 - 四倍体核型的人类胚胎的未培养的胚外组织的倍性水平进行了研究。仅13个胚胎（43%）被证实为四倍体，这为长期体外培养过程中胎盘细胞多倍体化的假说提供了证据。结果表明，四倍体细胞在内细胞团衍生物中的优先分隔与枯萎卵有关，枯萎卵是人类胚胎畸形发生最严重的类型。
