[39种HOX基因在食管癌细胞系中的表达]
[Expression of 39 HOX genes in esophageal cancer cell lines].
作者信息
Gu Zhen-dong, Chen Xiao-mei, Zhang Wei, Gu Jin, Chen Ke-neng
机构信息
Department of Thoracic Surgery, Peking University School of Oncology, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Beijing 100036, China.
出版信息
Zhonghua Wei Chang Wai Ke Za Zhi. 2007 Jul;10(4):365-7.
OBJECTIVE
To identify whether HOX genes can be used as markers of esophageal cancer.
METHODS
The expression of 39 HOX genes in esophageal cancer cell lines was examined. Specific primers were designed and RT- PCR was performed for each HOX gene members in above esophageal cancer cell lines, EC109 and CAES.
RESULTS
Fifteen out of 39 HOX genes were expressed in esophageal cancer cell lines. They were HOXA2, HOXA7, HOXA9, HOXA10, HOXA13, HOXB7, HOXB9, HOXC4, HOXC5, HOXC6, HOXC8, HOXC9, HOXD9, HOXD10, and HOXD13 respectively. Of them. Eleven genes were overlapped with the ones detected in human esophageal squamous cell carcinoma(ESCC) in our former study.
CONCLUSION
This study reconfirms our former that some HOX genes are deregulated expressed in ESCC, which provides more positive evidence for their roles in ESCC.
目的
确定HOX基因是否可作为食管癌的标志物。
方法
检测39个HOX基因在食管癌细胞系中的表达。设计特异性引物，对上述食管癌细胞系EC109和CAES中的每个HOX基因成员进行逆转录聚合酶链反应（RT-PCR）。
结果
39个HOX基因中有15个在食管癌细胞系中表达。它们分别是HOXA2、HOXA7、HOXA9、HOXA10、HOXA13、HOXB7、HOXB9、HOXC4、HOXC5、HOXC6、HOXC8、HOXC9、HOXD9、HOXD10和HOXD13。其中，11个基因与我们之前研究中在人食管鳞状细胞癌（ESCC）中检测到的基因重叠。
结论
本研究再次证实了我们之前的发现，即一些HOX基因在ESCC中表达失调，这为它们在ESCC中的作用提供了更多的阳性证据。
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