抗幽门螺杆菌抗体与人红细胞膜抗原的交叉反应性。
Cross-reactivity of anti-H pylori antibodies with membrane antigens of human erythrocytes.
作者信息
Guo Feng-Hua, Yan Xiao-Mei, Fan Chun-Xiang, Zhao Fei, Hu Yuan, Xiao Di, Zeng Xun, Zhang Mao-Jun, He Li-Hua, Meng Fan-Ling, Zhang Jian-Zhong
机构信息
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China.
出版信息
World J Gastroenterol. 2007 Jul 21;13(27):3742-6. doi: 10.3748/wjg.v13.i27.3742.
AIM
To investigate whether anti-H pylori antibodies have cross-reaction with antigens of erythrocyte membrane.
METHODS
Blood samples were collected from 14 volunteers (8 positive and 6 negative for H pylori detected by (13)C-urea breath test) of the general population. Erythrocyte membrane proteins of the subjects were examined by Western blot using anti-H pylori serum. The proteins related to the positive bands were identified by mass spectrum analysis.
RESULTS
Anti-H pylori antibodies had cross-reaction with the proteins of about 50 kDa of erythrocyte membranes in all samples independent of H pylori infection. One protein in the positive band was identified as Chain S, the crystal structure of the cytoplasmic domain of human erythrocyte Band-3 protein.
CONCLUSION
Anti-H pylori antibodies cross-react with some antigens of human erythrocyte membrane, which may provide a clue for the relationship between H pylori infection and vascular disorders.
目的
研究抗幽门螺杆菌抗体是否与红细胞膜抗原发生交叉反应。
方法
采集14名普通人群志愿者的血样（13C-尿素呼气试验检测幽门螺杆菌，8例阳性，6例阴性）。用抗幽门螺杆菌血清通过蛋白质印迹法检测受试者的红细胞膜蛋白。通过质谱分析鉴定与阳性条带相关的蛋白质。
结果
无论是否感染幽门螺杆菌，抗幽门螺杆菌抗体均与所有样本中约50 kDa的红细胞膜蛋白发生交叉反应。阳性条带中的一种蛋白质被鉴定为链S，即人红细胞带3蛋白胞质结构域的晶体结构。
结论
抗幽门螺杆菌抗体与人红细胞膜的某些抗原发生交叉反应，这可能为幽门螺杆菌感染与血管疾病之间的关系提供线索。
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