活体大脑中的乙醇氧化
Ethanol oxidation in the living brain.
作者信息
Zimatkin Sergey M, Buben Alexander L
机构信息
Grodno State Medical University, 80 Gorkogo Street, Grodno, 230015, Belarus.
出版信息
Alcohol Alcohol. 2007 Nov-Dec;42(6):529-32. doi: 10.1093/alcalc/agm059. Epub 2007 Jul 28.
AIM
The examination of the possibility of ethanol oxidation in the brain in vivo and the evaluation of the enzyme catalase in this process.
METHODS
We anesthetized rats and perfused the brain with ethanol solutions through the lateral ventricle and collected the perfusate from the Cisterna magna. We determined ethanol and acetaldehyde in the perfusate by gas chromatography.
RESULTS
It was found that the passage of ethanol solution (85 and 90 mM) through the ventricular system of the rat brain (6-43 microl/min) results in the significant (up to 98%) elimination of ethanol from the perfusing fluid and in the appearance of acetaldehyde (up-to 60 microM) in the perfusate. The addition of the catalase inhibitor, aminotriazole, (10 mM) to the perfusing fluid decreased ethanol elimination significantly.
CONCLUSIONS
The ethanol oxidation and AA accumulation take place in the living brain. The enzyme catalase is involved in this process.
目的
检测体内大脑中乙醇氧化的可能性，并评估该过程中过氧化氢酶的作用。
方法
将大鼠麻醉，通过侧脑室用乙醇溶液灌注大脑，并从枕大池收集灌注液。采用气相色谱法测定灌注液中的乙醇和乙醛。
结果
发现乙醇溶液（85和90 mM）以6 - 43微升/分钟的流速通过大鼠脑室内系统后，灌注液中的乙醇显著消除（高达98%），且灌注液中出现乙醛（高达60微摩尔）。向灌注液中添加过氧化氢酶抑制剂氨基三唑（10 mM）可显著降低乙醇的消除。
结论
乙醇氧化和乙醛积累发生在活体大脑中。过氧化氢酶参与了这一过程。
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