Kosaka Katsuko, Kosaka Toshio
School of Health Sciences, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan.
Brain Res. 2007 Sep 5;1167:42-55. doi: 10.1016/j.brainres.2007.04.087. Epub 2007 Jul 6.
We analyzed the cellular composition of the juxtaglomerular region in the main olfactory bulb of C57B/6J strain mice, focusing on 1) the compartmental organization of the glomerulus and the presence of type 1 and 2 periglomerular cells, 2) the colocalization relationships among the 4 major chemically identified groups of periglomerular cells, glutamic acid decarboxylase (GAD)/gamma-aminobutyric acid (GABA), tyrosine hydroxylase, calretinin and calbindin D28k positive periglomerular cells, and 3) the chemical properties of the nitric oxide synthase (NOS)-positive juxtaglomerular cells. We confirmed the compartmental organization of the glomerulus and the presence of both type 1 and 2 periglomerular cells in the mice. Similar to rat periglomerular cells, the tyrosine hydroxylase-positive cells were type 1 and GAD/GABA-positive. On the other hand, both the calbindin D28k-positive and calretinin-positive cells were type 2 periglomerular cells, but in contrast to those in rats, which are GAD/GABA-negative, all of the calbindin D28k-positive periglomerular cells and 65% of the calretinin-positive periglomerular cells were GAD/GABA-positive. The GAD/GABA-positive cells thus included both type 1 and type 2 periglomerular cells. Juxtaglomerular NOS-positive cells have been proposed as a subgroup of type 1 periglomerular cells that are separate from the calretinin-positive and calbindin D28k-positive cells in rats. However, in the mice, about 70% of the NOS-positive cells were calretinin-positive, and 50% of the calretinin-positive cells were NOS-positive. We herein reveal the significant species differences in the chemical properties of periglomerular cells and suggest that the cellular organization of the mouse main olfactory bulb cannot be extrapolated from that of rats.
我们分析了C57B/6J品系小鼠主嗅球中肾小球旁区域的细胞组成,重点关注:1)肾小球的分区组织以及1型和2型球周细胞的存在;2)4种主要化学鉴定的球周细胞群,即谷氨酸脱羧酶(GAD)/γ-氨基丁酸(GABA)、酪氨酸羟化酶、钙视网膜蛋白和钙结合蛋白D28k阳性球周细胞之间的共定位关系;3)一氧化氮合酶(NOS)阳性肾小球旁细胞的化学特性。我们证实了小鼠肾小球的分区组织以及1型和2型球周细胞的存在。与大鼠球周细胞相似,酪氨酸羟化酶阳性细胞为1型,GAD/GABA阳性。另一方面,钙结合蛋白D28k阳性和钙视网膜蛋白阳性细胞均为2型球周细胞,但与大鼠不同,大鼠的这些细胞为GAD/GABA阴性,而所有钙结合蛋白D28k阳性球周细胞和65%的钙视网膜蛋白阳性球周细胞为GAD/GABA阳性。因此,GAD/GABA阳性细胞包括1型和2型球周细胞。肾小球旁NOS阳性细胞被认为是大鼠1型球周细胞的一个亚群,与钙视网膜蛋白阳性和钙结合蛋白D28k阳性细胞不同。然而,在小鼠中,约70%的NOS阳性细胞为钙视网膜蛋白阳性,50%的钙视网膜蛋白阳性细胞为NOS阳性。我们在此揭示了球周细胞化学特性的显著种属差异,并表明不能从小鼠主嗅球的细胞组织推断大鼠的细胞组织。