Lange Clemens, Ehlken Christoph, Martin Gottfried, Konzok Kirstin, Moscoso Del Prado Jaime, Hansen Lutz L, Agostini Hansjürgen T
Augenklinik, Universitätsklinikum Freiburg, Killianstrasse 5, 79106 Freiburg, Germany.
Exp Eye Res. 2007 Sep;85(3):323-7. doi: 10.1016/j.exer.2007.05.007. Epub 2007 Jun 13.
The effect of the heparin analog 5-amino-2-naphthalenesulfonate (5-amino-2-NMS) on retinal neovascularization was investigated in the mouse model for oxygen-induced retinopathy (OIR). From postnatal day 7 (P7) until P12, mice were kept in a 75% oxygen environment. On P12, they received an intravitreal injection of 10mM 5-amino-2-NMS in one eye and PBS as control substance in the fellow eye. The animals were intracardially perfused with fluorescein-dextran solution on P17. Retinal whole mounts were prepared and ischemic retinopathy was evaluated in 30 animals using a standardized retinopathy score. A single intravitreal injection of 5-amino-2-NMS reduces significantly angioproliferative changes (blood vessel tufts, extra-retinal neovascularization, and blood vessel tortuosity) compared to the contralateral control eye (p=0.025). The median retinopathy score (maximal 13) for the 5-amino-2-NMS treated eyes was 6 versus 8 for the control eyes. 5-Amino-2-NMS binds to the heparin-binding site of FGF1 and FGF2 and thus may be a promising substance for the local treatment of retinal neovascularization.
在氧诱导性视网膜病变（OIR）小鼠模型中，研究了肝素类似物5-氨基-2-萘磺酸盐（5-氨基-2-NMS）对视网膜新生血管形成的影响。从出生后第7天（P7）到P12，将小鼠置于75%氧气环境中。在P12时，给一只眼睛玻璃体内注射10mM 5-氨基-2-NMS，另一只眼睛注射PBS作为对照物质。在P17时，对动物进行心脏灌注荧光素-葡聚糖溶液。制备视网膜全层铺片，并使用标准化视网膜病变评分对30只动物的缺血性视网膜病变进行评估。与对侧对照眼相比，单次玻璃体内注射5-氨基-2-NMS可显著降低血管增殖性变化（血管丛、视网膜外新生血管形成和血管迂曲）（p=0.025）。5-氨基-2-NMS治疗组眼睛的视网膜病变评分中位数（最高13分）为6分，而对照组为8分。5-氨基-2-NMS与FGF1和FGF2的肝素结合位点结合，因此可能是局部治疗视网膜新生血管形成的一种有前景的物质。
