Hodges E, Stacey G, White D, Howell W, Smith J
Regional Immunology Service, Southampton General Hospital, U.K.
Leuk Res. 1991;15(12):1117-24. doi: 10.1016/0145-2126(91)90179-w.
Marrow involvement in 20 patients with non-Hodgkin's lymphoma (NHL) were studied by histology, immunophenotypic and genotypic methods. Eighteen of these trephines were histologically involved with recognizable lymphomatous infiltrates and five of these were the primary disease site. In the remaining two cases (with histologically involved lymph nodes) the trephines were uninvolved with tumour. Three B-cell cases expressing surface immunoglobulin (sIg) and/or CD37 and one case not analysed phenotypically showed Ig gene rearrangements. The two remaining cases with B NHL showed no gene rearrangements, however, in one of these the trephine was histologically uninvolved with tumour. Twelve out of 14 T-cell cases were characterized by variable or absent expression of one or more T-cell antigens from the tumour population, one case was negative for all T-cell antigens and the remaining case was not histologically involved with tumour. All three lymphoblastic lymphomas and only 4/11 peripheral T-cell lymphomas (PTCL) cases revealed T-cell receptor (TcR) gene rearrangements. One of the latter cases also exhibited Ig JH gene rearrangements. This study demonstrates the usefulness of bone marrow trephines (BMT) in histologic, phenotypic and genotypic analyses. However, although genotypic data confirm clonality in B NHL and the lymphoblastic lymphomas there was genotypic heterogeneity within the PTCL group.
采用组织学、免疫表型和基因分型方法研究了20例非霍奇金淋巴瘤（NHL）患者的骨髓受累情况。其中18例骨髓活检组织学显示有可识别的淋巴瘤浸润，5例为原发疾病部位。其余2例（淋巴结组织学受累）骨髓未发现肿瘤。3例表达表面免疫球蛋白（sIg）和/或CD37的B细胞病例以及1例未进行表型分析的病例显示Ig基因重排。其余2例B NHL病例未显示基因重排，不过其中1例骨髓组织学未发现肿瘤。14例T细胞病例中，12例表现为肿瘤细胞群中一种或多种T细胞抗原表达可变或缺失，1例所有T细胞抗原均为阴性，其余1例骨髓组织学未发现肿瘤。所有3例淋巴母细胞淋巴瘤以及仅4/11例外周T细胞淋巴瘤（PTCL）病例显示T细胞受体（TcR）基因重排。后者中的1例还表现出Ig JH基因重排。本研究证明了骨髓活检（BMT）在组织学、表型和基因分型分析中的有用性。然而，尽管基因分型数据证实了B NHL和淋巴母细胞淋巴瘤中的克隆性，但PTCL组内存在基因异质性。
