Stricker Astrid R, Steiger Matthias G, Mach Robert L
Gene Technology, Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9/166/5/2, A-1060 Wien, Austria.
FEBS Lett. 2007 Aug 21;581(21):3915-20. doi: 10.1016/j.febslet.2007.07.025. Epub 2007 Jul 23.
This study reports the vital regulatory influence of Xyr1 (xylanase regulator 1) on the transcription of hydrolytic enzyme-encoding genes and hydrolase formation on lactose in Hypocrea jecorina. While the transcription of the xyr1 gene itself is achieved by release of carbon catabolite repression, the transcript formation of xyn1 (xylanase 1) is regulated by an additional induction mechanism mediated by lactose. Xyr1 has an important impact on lactose metabolism by directly activating xyl1 (xylose reductase 1) transcription and indirectly influencing transcription of bga1 (beta-galactosidase 1). The latter is achieved by regulating the conversion of D-galactose to the inducing carbon source galactitol.
本研究报道了木聚糖酶调节因子1（Xyr1）对里氏木霉中水解酶编码基因转录以及乳糖水解酶形成的重要调控作用。虽然xyr1基因自身的转录是通过解除碳分解代谢物阻遏来实现的，但木聚糖酶1（xyn1）的转录本形成是由乳糖介导的另一种诱导机制调控的。Xyr1通过直接激活木糖还原酶1（xyl1）转录并间接影响β-半乳糖苷酶1（bga1）转录，对乳糖代谢产生重要影响。后者是通过调节D-半乳糖向诱导性碳源半乳糖醇的转化来实现的。
