肿瘤坏死因子依赖性小鼠主动脉弓动脉粥样硬化壁增厚从早期到晚期的差异进展的磁共振成像随访
MRI follow-up of TNF-dependent differential progression of atherosclerotic wall-thickening in mouse aortic arch from early to advanced stages.
作者信息
Kober Frank, Canault Matthias, Peiretti Franck, Mueller Christoph, Kopp Francis, Alessi Marie-Christine, Cozzone Patrick J, Nalbone Gilles, Bernard Monique
机构信息
Centre de Résonance Magnétique Biologique et Médicale, CNRS UMR n 6612, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard J. Moulin, 13005 Marseille, France.
出版信息
Atherosclerosis. 2007 Dec;195(2):e93-9. doi: 10.1016/j.atherosclerosis.2007.06.015. Epub 2007 Jul 30.
OBJECTIVES
An optimized, longitudinal in vivo magnetic resonance vessel wall-imaging protocol was evaluated regarding its capability of detecting differences in the time-dependent atherosclerotic lesion progression in the aortic arch between ApoE(-/-) and double-deficient ApoE(-/-)/TNF(-/-) mice at comparatively early plaque development stages.
MATERIALS AND METHODS
Seven ApoE(-/-) and seven ApoE(-/-)/TNF(-/-) female mice underwent MRI at 11.75 teslas at four stages up to 26 weeks of age. A double-gated spin-echo MRI sequence was used with careful perpendicular slice positioning to visualize the vessel wall of the ascending aortic arch.
RESULTS
Wall-thickness progression measured with MRI was significant at 11 weeks of age in ApoE(-/-) mice, but only at 26 weeks in ApoE(-/-)/TNF(-/-) mice. A significant correlation was found between MRI wall-thickness and lesion area determined on histology.
CONCLUSION
MRI was shown to be sensitive enough to reveal subtle genetically-induced differences in lesion progression at ages earlier than 25 weeks.
目的
评估一种优化的纵向体内磁共振血管壁成像方案，该方案用于检测在相对早期斑块发展阶段，载脂蛋白E基因敲除（ApoE(-/-)）小鼠与双基因敲除（ApoE(-/-)/肿瘤坏死因子基因敲除（TNF(-/-)））小鼠主动脉弓中随时间变化的动脉粥样硬化病变进展差异的能力。
材料与方法
7只ApoE(-/-)雌性小鼠和7只ApoE(-/-)/TNF(-/-)雌性小鼠在11.75特斯拉磁场下，直至26周龄的四个阶段接受磁共振成像（MRI）检查。使用双门控自旋回波MRI序列，并仔细进行垂直切片定位，以可视化升主动脉弓的血管壁。
结果
MRI测量的ApoE(-/-)小鼠在11周龄时壁厚进展显著，但ApoE(-/-)/TNF(-/-)小鼠仅在26周龄时壁厚进展显著。MRI测量的壁厚与组织学确定的病变面积之间存在显著相关性。
结论
MRI显示出足够的敏感性，能够在25周龄之前揭示基因诱导的病变进展细微差异。
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