Kober Frank, Canault Matthias, Peiretti Franck, Mueller Christoph, Kopp Francis, Alessi Marie-Christine, Cozzone Patrick J, Nalbone Gilles, Bernard Monique
Centre de Résonance Magnétique Biologique et Médicale, CNRS UMR n 6612, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard J. Moulin, 13005 Marseille, France.
Atherosclerosis. 2007 Dec;195(2):e93-9. doi: 10.1016/j.atherosclerosis.2007.06.015. Epub 2007 Jul 30.
An optimized, longitudinal in vivo magnetic resonance vessel wall-imaging protocol was evaluated regarding its capability of detecting differences in the time-dependent atherosclerotic lesion progression in the aortic arch between ApoE(-/-) and double-deficient ApoE(-/-)/TNF(-/-) mice at comparatively early plaque development stages.
Seven ApoE(-/-) and seven ApoE(-/-)/TNF(-/-) female mice underwent MRI at 11.75 teslas at four stages up to 26 weeks of age. A double-gated spin-echo MRI sequence was used with careful perpendicular slice positioning to visualize the vessel wall of the ascending aortic arch.
Wall-thickness progression measured with MRI was significant at 11 weeks of age in ApoE(-/-) mice, but only at 26 weeks in ApoE(-/-)/TNF(-/-) mice. A significant correlation was found between MRI wall-thickness and lesion area determined on histology.
MRI was shown to be sensitive enough to reveal subtle genetically-induced differences in lesion progression at ages earlier than 25 weeks.
评估一种优化的纵向体内磁共振血管壁成像方案，该方案用于检测在相对早期斑块发展阶段，载脂蛋白E基因敲除（ApoE(-/-)）小鼠与双基因敲除（ApoE(-/-)/肿瘤坏死因子基因敲除（TNF(-/-)））小鼠主动脉弓中随时间变化的动脉粥样硬化病变进展差异的能力。
7只ApoE(-/-)雌性小鼠和7只ApoE(-/-)/TNF(-/-)雌性小鼠在11.75特斯拉磁场下，直至26周龄的四个阶段接受磁共振成像（MRI）检查。使用双门控自旋回波MRI序列，并仔细进行垂直切片定位，以可视化升主动脉弓的血管壁。
MRI测量的ApoE(-/-)小鼠在11周龄时壁厚进展显著，但ApoE(-/-)/TNF(-/-)小鼠仅在26周龄时壁厚进展显著。MRI测量的壁厚与组织学确定的病变面积之间存在显著相关性。
MRI显示出足够的敏感性，能够在25周龄之前揭示基因诱导的病变进展细微差异。
