Zhu Yu, Xie Liping, Chen Guang, Wang Hongzhong, Zhang Rongqing
Department of Biological Science and Biotechnology, Tsinghua University, Beijing, China.
Pharmazie. 2007 Jun;62(6):439-44.
Oridonin, an active ditepenoid component isolated from Rabdosia rubescens which is currently one of the most important Chinese traditional herbs, has been reported to exhibit anti-tumor effects in vitro. In this study, the anti-proliferation effect of oridonin against the human colorectal carcinoma cells HT29 was investigated both in vitro and in vivo. MTT assay showed that oridonin inhibited HT29 cells in a time- and dose-dependent manner. Flow cytometric analysis demonstrated that oridonin induced a G2/M phase arrest. Apoptotic bodies were observed by Hoechst 32258 fluorescence staining. Notable apoptosis and decrease of mitochondrial membrane potentials was also detected by flow cytometry. In the in vivo experiments, oridonin (10, 15, 20 mg kg(-1) of body weight, on days 1-12) was injected intraperitoneally into mice 24 h after the mice were incubated with HT29 cells. Inhibition of the solid tumor was observed. As a result, oridonin could inhibit the proliferation of HT29 cells both in vitro and in vivo, and induce apoptosis partly via the mitochondrial pathway.
冬凌草甲素是从冬凌草中分离出的一种活性二萜类成分，冬凌草是目前最重要的传统中药之一，据报道其在体外具有抗肿瘤作用。在本研究中，对冬凌草甲素在体外和体内对人结肠癌细胞HT29的抗增殖作用进行了研究。MTT法显示冬凌草甲素以时间和剂量依赖性方式抑制HT29细胞。流式细胞术分析表明，冬凌草甲素诱导细胞阻滞于G2/M期。通过Hoechst 32258荧光染色观察到凋亡小体。通过流式细胞术还检测到明显的细胞凋亡和线粒体膜电位降低。在体内实验中，在小鼠接种HT29细胞24小时后，腹腔注射冬凌草甲素（10、15、20 mg·kg⁻¹体重，第1 - 12天）。观察到实体瘤受到抑制。结果表明，冬凌草甲素在体外和体内均可抑制HT29细胞的增殖，并部分通过线粒体途径诱导细胞凋亡。
