Schäfer R, Nirkko A C, Ambühl P M, Grzeschik K H, Schwarte-Waldhoff I
Division of Cancer Research, Institute of Pathology, University of Zürich, Switzerland.
Oncogene. 1991 Dec;6(12):2221-8.
Genetic suppression of the neoplastic phenotype has been demonstrated in somatic cell hybrids between tumor and normal cells. Suppression in whole-cell and microcell hybrids cannot, as yet, be attributed to specific elements defined at the molecular level. To identify a gene capable of suppressing the neoplastic phenotype, we have introduced DNA of normal human cells into tumorigenic Chinese hamster Wg3-h-o cells. Primary and secondary transfectants which exhibit the suppressed phenotype similar to Wg3-h-o x embryonic fibroblast hybrids were selected. The cells require serum growth factors and anchorage for proliferation in vitro and show a reduced tumorigenicity in nude mice. Transferred human DNA segments were molecularly cloned from a secondary transfectant. Indirect evidence suggests that the cloned human DNA is associated with the expression of the suppressed phenotype.
肿瘤细胞与正常细胞之间的体细胞杂交已证明可对肿瘤表型进行基因抑制。目前，全细胞杂交和微细胞杂交中的抑制作用还不能归因于分子水平上明确的特定元件。为了鉴定一个能够抑制肿瘤表型的基因，我们已将正常人细胞的DNA导入致瘤性中国仓鼠Wg3-h-o细胞中。选择了表现出与Wg3-h-o×胚胎成纤维细胞杂交体相似的抑制表型的原代和二代转染子。这些细胞在体外增殖需要血清生长因子和贴壁，并且在裸鼠中致瘤性降低。从一个二代转染子中对转移的人类DNA片段进行了分子克隆。间接证据表明，克隆的人类DNA与抑制表型的表达相关。
