Wang Xiaodan, Yamaguchi Nobuyasu, Someya Takashi, Nasu Masao
Environmental Science and Microbiology, Graduate School of Pharmaceutical Science, Osaka University, 1-6, Yamada-oka, Suita, Osaka 565-0871, Japan.
J Microbiol Methods. 2007 Oct;71(1):1-6. doi: 10.1016/j.mimet.2007.06.019. Epub 2007 Jul 6.
The micro-colony method was used to enumerate viable bacteria in composts. Cells were vacuum-filtered onto polycarbonate filters and incubated for 18 h on LB medium at 37 degrees C. Bacteria on the filters were stained with SYBR Green II, and enumerated using a newly developed micro-colony auto counting system which can automatically count micro-colonies on half the area of the filter within 90 s. A large number of bacteria in samples retained physiological activity and formed micro-colonies within 18 h, whereas most could not form large colonies on conventional media within 1 week. The results showed that this convenient technique can enumerate viable bacteria in compost rapidly for its efficient quality control.
采用微菌落法对堆肥中的活菌进行计数。将细胞真空过滤到聚碳酸酯滤膜上，在37℃的LB培养基上培养18小时。滤膜上的细菌用SYBR Green II染色，并用新开发的微菌落自动计数系统进行计数，该系统可在90秒内自动对滤膜一半面积上的微菌落进行计数。样品中的大量细菌保持生理活性，并在18小时内形成微菌落，而大多数细菌在1周内无法在传统培养基上形成大菌落。结果表明，这种便捷的技术能够快速对堆肥中的活菌进行计数，以实现高效的质量控制。
