Momose-Sato Yoko, Kinoshita Masae, Sato Katsushige
Department of Physiology, Tokyo Medical and Dental University Graduate School and Faculty of Medicine, 1-5-45 Yushima, Tokyo 113-8519, Japan.
Neurosci Lett. 2007 Aug 16;423(2):138-42. doi: 10.1016/j.neulet.2007.07.001. Epub 2007 Jul 31.
Using voltage-sensitive dye recording, we traced the ontogenetic expression of neural excitability related to the glossopharyngeal nerve (N. IX) and the vagus nerve (N. X) in the embryonic chick brainstem. At the 3.5-day embryonic stage, by averaging optical signals, we succeeded in recording very small action potential-related optical responses (DeltaI/I<10(-4)) induced by N. IX stimulation in the nucleus of the glossopharyngeal nerve (motor nucleus). This suggests that glossopharyngeal excitability in the motor nucleus is first generated no later than this developmental stage. On the other hand, action potential-related optical responses induced by N. X stimulation were first detected at the 4-day embryonic stage. Comparison with morphogenesis indicated that glossopharyngeal and vagal motoneurons acquire their excitability and send their axons to the periphery soon after they leave the proliferative pool.
利用电压敏感染料记录技术，我们追踪了胚胎期鸡脑干中与舌咽神经（IX 神经）和迷走神经（X 神经）相关的神经兴奋性的个体发育表达。在胚胎 3.5 天阶段，通过对光信号进行平均，我们成功记录到了由舌咽神经刺激在舌咽神经核（运动核）中诱发的非常小的与动作电位相关的光反应（ΔI/I<10(-4)）。这表明运动核中的舌咽神经兴奋性最早在这个发育阶段就已产生。另一方面，由 X 神经刺激诱发的与动作电位相关的光反应最早在胚胎 4 天阶段被检测到。与形态发生的比较表明，舌咽和迷走运动神经元在离开增殖池后不久就获得了兴奋性并将其轴突发送到外周。
