对北卡罗来纳州哮喘儿童家中灰尘中霉菌的定量聚合酶链反应分析。
Quantitative PCR analysis of molds in the dust from homes of asthmatic children in North Carolina.
作者信息
Vesper Stephen, McKinstry Craig, Ashley Peter, Haugland Richard, Yeatts Karin, Bradham Karen, Svendsen Erik
机构信息
National Exposure Research Laboratory (NERL), United States (US) Environmental Protection Agency, 26 West M. L. King Ave., M. L. 314, Cincinnati, 45268, Ohio, USA.
出版信息
J Environ Monit. 2007 Aug;9(8):826-30. doi: 10.1039/b704359g. Epub 2007 Jul 10.
The vacuum bag (VB) dust from the homes of 19 asthmatic children in North Carolina (NC) was analyzed by mold specific quantitative PCR. These results were compared to the analysis of the VB dust from 176 homes in the HUD, American Healthy Home Survey of homes in the US. The Environmental Relative Moldiness Index (ERMI) was calculated for each of the homes. The mean and standard deviation (SD) of the ERMI values in the homes of the NC asthmatic children was 16.4 (6.77), compared to the HUD survey VB ERMI value mean and SD of 11.2 (6.72), and was significantly greater (t-test, p = 0.003) in the NC asthmatic children's homes. The molds Chaetomium globosum, Aspergillus fumigatus, and the Eurotium Group were the primary species in the NC homes of asthmatics, making the ERMI values significantly higher (p < 0.02 for each). Vacuum bag dust analysis may be a useful method for estimating the mold burden in a home.
通过霉菌特异性定量PCR分析了北卡罗来纳州（NC）19名哮喘儿童家中的真空袋（VB）灰尘。将这些结果与美国住房和城市发展部（HUD）对美国176户家庭进行的美国健康家庭调查中VB灰尘的分析结果进行了比较。计算了每户家庭的环境相对霉菌指数（ERMI）。北卡罗来纳州哮喘儿童家庭中ERMI值的平均值和标准差（SD）为16.4（6.77），相比之下，HUD调查中VB的ERMI值平均值和标准差为11.2（6.72），且北卡罗来纳州哮喘儿童家庭中的该值显著更高（t检验，p = 0.003）。球毛壳菌、烟曲霉和散囊菌属是北卡罗来纳州哮喘患者家中的主要霉菌种类，使得ERMI值显著更高（每种均p < 0.02）。真空袋灰尘分析可能是估计家庭中霉菌负荷的一种有用方法。
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