Liu Dan, Tang Hai-feng, Zhang San-qi, Ding Yu, Yang Chun-e
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(11):1019-24.
To study the technological parameters of the purification process for effective part from Polygonum cuspidatum.
Using adsorption capacities and desorption rates of polydatin, resveratrol,emodin,physcion and total anthraquinone as the primary screening indexes, six resins were surveyed,and the optimized conditions of adsorption and desorption of the effective ingredients were studied.
Resin D101 gave good separation performance and was selected to purify the effective part in Polygonum cuspidatum. The optimum parameters were established as the following: 1 BV (bed volume) sample extract was passed through the column with a flow rate of 2.4 BV x h(-1), 30 min later,the column was washed with 2 BV water, 2 BV 20% ethanol, 5 BV 50% ethanol, 2 BV 70% ethanol and 5 BV 95% ethanol, respectively. The combined 50% and 95% ethanolic elutes were concentrated to yield the purified effctive part.
The purity of the total effective ingredients in the product was up to 36. 87%. Macroporous resin D101 could be well used in separating and purifying the effective part from Polygonum cuspidatum.
研究虎杖有效部位纯化工艺的技术参数。
以虎杖苷、白藜芦醇、大黄素、大黄酚及总蒽醌的吸附量和解吸率为主要筛选指标，考察6种树脂，并研究有效成分吸附和解吸的优化条件。
D101树脂分离性能良好，选用其纯化虎杖有效部位。确定最佳参数如下：1倍床体积（BV）的样品提取物以2.4 BV×h-1的流速通过柱子，30分钟后，分别用2 BV水、2 BV 20%乙醇、5 BV 50%乙醇、2 BV 70%乙醇和5 BV 95%乙醇冲洗柱子。合并50%和95%乙醇洗脱液，浓缩得到纯化的有效部位。
产品中总有效成分的纯度达36.87%。大孔树脂D101可很好地用于虎杖有效部位的分离纯化。
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