大孔树脂纯化唐古特红景天总黄酮工艺的初步研究
[Preliminary study on purification technics of total flavonoids in Rhodiola dumulosa by macroporous resin].
作者信息
Tan Lin, Guo Zeng-Jun, Xu Ying
机构信息
Department of Pharmacy, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
出版信息
Zhong Yao Cai. 2008 Nov;31(11):1740-3.
OBJECTIVE
To choose the best macroporous resin for purifying total flavonoids in Rhodiola dumulosa and study various factors affecting purification of resin in order to optimize the technics.
METHODS
Using methods combining static adsorption with desorption and dynamic adsorption with desorption, evaluating the technics by determination of total flavonoids in Rhodiola dumulosa using ultraviolet and visible spectrophotometry.
RESULTS
The best macroporous resin was D101. The optimized technics were as follows: sample total flavonoids concentration was 3.625 mg/ml, adsorption velocity was 2 BV/h, desorption ethanol concentration was 95%, desorption velocity was 2 BV/h, eluant volume was 5 BV, the purity of total flavonoids could reach 48.5%.
CONCLUSIONS
Purification performance of D101 macroporous resin is the best, and it is fit for the preliminary purification of total flavonoids in Rhodiola dumulosa.
目的
筛选出用于纯化小丛红景天总黄酮的最佳大孔树脂，并研究影响树脂纯化的各种因素，以优化工艺。
方法
采用静态吸附与解吸、动态吸附与解吸相结合的方法，通过紫外可见分光光度法测定小丛红景天中总黄酮的含量来评价工艺。
结果
最佳大孔树脂为D101。优化工艺如下：样品总黄酮浓度为3.625mg/ml，吸附流速为2BV/h，解吸乙醇浓度为95%，解吸流速为2BV/h，洗脱剂体积为5BV，总黄酮纯度可达48.5%。
结论
D101大孔树脂纯化性能最佳，适合小丛红景天总黄酮的初步纯化。
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