[Preliminary study on purification technics of total flavonoids in Rhodiola dumulosa by macroporous resin].

OBJECTIVE

To choose the best macroporous resin for purifying total flavonoids in Rhodiola dumulosa and study various factors affecting purification of resin in order to optimize the technics.

METHODS

Using methods combining static adsorption with desorption and dynamic adsorption with desorption, evaluating the technics by determination of total flavonoids in Rhodiola dumulosa using ultraviolet and visible spectrophotometry.

RESULTS

The best macroporous resin was D101. The optimized technics were as follows: sample total flavonoids concentration was 3.625 mg/ml, adsorption velocity was 2 BV/h, desorption ethanol concentration was 95%, desorption velocity was 2 BV/h, eluant volume was 5 BV, the purity of total flavonoids could reach 48.5%.

CONCLUSIONS

Purification performance of D101 macroporous resin is the best, and it is fit for the preliminary purification of total flavonoids in Rhodiola dumulosa.