Qiao Yu-Huan, Cheng Jia, Guo Rui-Xia
Department of Obstetrics and Gynecology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Fu Chan Ke Za Zhi. 2007 May;42(5):325-9.
To analyze the expression of phosphorylated protein kinase B (pAKT) and PTEN protein in ovarian epithelial cancer and to investigate the correlations between their expression and prognosis of ovarian epithelial cancers.
Expression of pAKT and PTEN in 12 normal ovarian tissues, 20 benign tumors, 12 borderline tumors and 80 cases of ovarian epithelial cancers were detected by immunohistochemical method, and their correlations were analyzed.
The positive expression of pAKT in normal ovarian and benign tumor tissues were significantly lower than that in ovarian epithelial cancers (8%, 10% vs 55%; P < 0.01), respectively. However, loss of PTEN expression in ovarian epithelial cancers was significantly higher than that in normal ovarian and benign tumor tissues, and the positive rate of PTEN expression were respectively, 45%, 100%, and 80% (P < 0.01). The expression of pAKT and PTEN were correlated with clinical stages, differentiation degree of cancer cells, and metastasis (including lymph node; P < 0.05). But there was no difference with regard to age, histological type and ascites. In ovarian cancers, a negative correlation between expression of pAKT and PTEN was observed (r = -0.444, P < 0.01). A univariate analysis revealed that clinical stage, differentiation degree, lymph node involvement, distant metastasis, pAKT and PTEN expression were correlative factors with prognosis (P < 0.05). Multivariate Cox analysis showed that PTEN and clinical stage were the independent risk factors of prognosis (P < 0.05).
The overexpression of pAKT and absence of PTEN are related to ovarian carcinogenesis and development. Loss of PTEN expression is the independent risk factor of poor prognosis in patients with ovarian epithelial cancers.
分析磷酸化蛋白激酶B（pAKT）和PTEN蛋白在卵巢上皮癌中的表达情况，并探讨其表达与卵巢上皮癌预后的相关性。
采用免疫组织化学方法检测12例正常卵巢组织、20例良性肿瘤、12例交界性肿瘤及80例卵巢上皮癌中pAKT和PTEN的表达，并分析其相关性。
正常卵巢组织和良性肿瘤组织中pAKT的阳性表达率显著低于卵巢上皮癌（分别为8%、10% vs 55%；P < 0.01）。然而，卵巢上皮癌中PTEN表达缺失显著高于正常卵巢组织和良性肿瘤组织，PTEN表达阳性率分别为45%、100%和80%（P < 0.01）。pAKT和PTEN的表达与临床分期、癌细胞分化程度及转移（包括淋巴结转移；P < 0.05）相关。但在年龄、组织学类型及腹水方面无差异。在卵巢癌中，观察到pAKT和PTEN表达呈负相关（r = -0.444，P < 0.01）。单因素分析显示，临床分期、分化程度、淋巴结受累、远处转移、pAKT和PTEN表达是与预后相关的因素（P < 0.05）。多因素Cox分析表明，PTEN和临床分期是预后的独立危险因素（P < 0.05）。
pAKT的过表达和PTEN的缺失与卵巢癌的发生发展有关。PTEN表达缺失是卵巢上皮癌患者预后不良的独立危险因素。
