银杏叶提取物EGb 761对H2O2诱导的RIN-mβ细胞凋亡的影响
[Effects of EGb 761 on the cell apoptosis induced by H2O2 in RIN-m beta cells].
作者信息
Ye Chun-ling, Jin Yong-liang, Ye Kai-he, Qin Liang
机构信息
Department of Pharmacology, Pharmacy College of Jinan University, Guangzhou 510632, China.
出版信息
Zhong Yao Cai. 2007 Apr;30(4):424-8.
OBJECTIVE
To investigate the effects of ginkgo biloba extract (EGb 761) on apoptosis induced by hydrogen peroxide (H2O2) in RIN-m beta-cells.
METHODS
The apoptotic model was made by H2O2 exposed for six hours with a concentration of 500 micromol/L The cytotoxicity was measured by MTT. Hoechst 33258 fluorescent staining were used to detect the protective effect of EGb 761 on the apoptosis of RIN-m beta-cells induced by H2O2. Annexin V-PI double staining of Flow cytometry were used to detect apoptosis quantitively.
RESULTS
Compare to control group, after exposed to 500 micromol/L H2O2 for 6 hours, the apoptosis rate incereased and cell survival rate were decreased considerably (P < 0.01). Pretreated for 10 hours with EGb 761, the flow cytometry results showed that the apoptosis rate decreased and cell survival rate were increased considerably (P < 0.01, compared to H2O2 control group).
CONCLUSION
EGb 761 can decrease RIN-m beta-cells damage and apoptosis induced by H2O2.
目的
研究银杏叶提取物（EGb 761）对过氧化氢（H2O2）诱导的RIN-mβ细胞凋亡的影响。
方法
用浓度为500微摩尔/升的H2O2处理6小时建立凋亡模型。采用MTT法检测细胞毒性。用Hoechst 33258荧光染色检测EGb 761对H2O2诱导的RIN-mβ细胞凋亡的保护作用。采用流式细胞术Annexin V-PI双染色法定量检测细胞凋亡。
结果
与对照组相比，500微摩尔/升H2O2处理6小时后，细胞凋亡率增加，细胞存活率显著降低（P<0.01）。用EGb 761预处理10小时，流式细胞术结果显示细胞凋亡率降低，细胞存活率显著增加（与H2O2对照组相比，P<0.01）。
结论
EGb 761可减轻H2O2诱导的RIN-mβ细胞损伤和凋亡。
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