丹酚酸B与银杏叶提取物EGb 761对β-淀粉样肽神经毒性抑制活性的比较

[Comparison of the inhibitory activities of salvianolic acid B and Ginkgo biloba extract EGb 761 on neurotoxicity of beta-amyloid peptide].

作者信息

Liu Chang-suo, Hu Jin-feng, Chen Nai-hong, Zhang Jun-tian

机构信息

Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

出版信息

Yao Xue Xue Bao. 2006 Aug;41(8):706-11.

PMID:17039773

Abstract

AIM

To compare the effects of salvianolic acid B (Sal B) and Ginkgo biloba extract EGb 761 on beta-amyloid peptide (beta-AP) fibril formation and cytotoxicity to PC12 cells.

METHODS

The inhibitory effects of Sal B and EGb 761 on beta-AP1-40 fibril formation were determined by using fluorescence analysis with Thioflavin T (ThT) and electron microscopic image. beta-AP25-35 was aged by incubating at 37 degrees C for 7 d, then the protein was incubated with PC12 cells. The protective effects of Sal B and EGb 761 against cytotoxicity induced by aged beta-AP25-35 in PC12 cells were evaluated by MTT reduction assay and flow cytometric analysis. beta-AP25-35-induced accumulation of intracellular reactive oxygen species (ROS) was determined by fluorescence analysis.

RESULTS

Both Sal B and EGb 761 inhibited the formation of amyloid fibrils, protected PC12 cells from beta-AP25-35-induced cytotoxicity, and decreased ROS accumulation caused by beta-AP25-35. The effective doses of Sal B were far lower than those of EGb 761.

CONCLUSION

Sal B was much more efficient than EGb 761 in inhibiting beta-AP aggregation and in protecting PC12 cells from beta-AP-induced cytotoxicity.

摘要

目的

比较丹酚酸B（Sal B）与银杏叶提取物EGb 761对β-淀粉样肽（β-AP）纤维形成及对PC12细胞细胞毒性的影响。

方法

采用硫黄素T（ThT）荧光分析和电子显微镜图像观察法，测定Sal B和EGb 761对β-AP1-40纤维形成的抑制作用。β-AP25-35在37℃孵育7天进行老化，然后将该蛋白与PC12细胞共同孵育。通过MTT还原试验和流式细胞术分析，评估Sal B和EGb 761对老化的β-AP25-35诱导的PC12细胞细胞毒性的保护作用。通过荧光分析测定β-AP25-35诱导的细胞内活性氧（ROS）积累。