Kim Ki-Sun, Choi Woo-Hyung, Choi Bo-Ra, Oh Sangtaek, Yea Sung Su, Yoon Moon-Young, Kim Dong-Eun
Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Republic of Korea.
FEBS Lett. 2007 Aug 21;581(21):4065-72. doi: 10.1016/j.febslet.2007.07.048. Epub 2007 Jul 30.
Self-replication process of the RNA ligase ribozyme molecules was investigated by using the modified RNA ligase ribozyme under alternating temperature condition that enhances turnover rate of the RNA ligation reaction. In our experiment, the RNA ligase ribozyme system mainly undergoes a cross-catalytic replication process, in which two ribozymes catalyze each other's synthesis from a total of four RNA substrates under alternating temperature condition, resulting in time-dependent accumulation of additional copies of the starting ribozymes in a reaction mixture. The present study demonstrates that cross-catalytic replication in nucleic acids system can be efficiently devised under the alternating temperature condition.
通过使用修饰的RNA连接酶核酶，在交替温度条件下研究了RNA连接酶核酶分子的自我复制过程，该条件提高了RNA连接反应的周转率。在我们的实验中，RNA连接酶核酶系统主要经历交叉催化复制过程，其中两个核酶在交替温度条件下从总共四个RNA底物催化彼此的合成，导致反应混合物中起始核酶的额外拷贝随时间积累。本研究表明，在交替温度条件下可以有效地设计核酸系统中的交叉催化复制。
