Bohgaki Miyako, Kitaguchi Hiromichi
Department of Medical Technology, Kobe Tokiwa College, Kobe, Japan.
Int J Hematol. 2007 Jul;86(1):42-8. doi: 10.1532/IJH97.06217.
When culturing human umbilical vein endothelial cells in a culture medium containing 4% human serum albumin, it was possible to maintain the epithelioid morphology and function for several months without subculturing. When coculturing endothelial cells and labeled monocytes/macrophages (Mo/Phi) that were collected from peripheral blood and allowed to engulf fluorescent latex beads, some Mo/Phi changed their shapes and became epithelioid cells that were indistinguishable from vascular endothelial cells. This transformation started within several hours of coculturing. At 7 days after the start of coculturing, more than half of the labeled cells were identified as endothelial-like cells morphologically. Furthermore, morphologically altered Mo/Phi did not express Mo/Phi-specific antigens, ie, the MHC Class II molecule and CD68, but expressed VE cadherin and vWF, which are specific antigens for endothelial cells, and labeled cells that changed into endothelial-like cells no longer engulfed fluorescent latex beads. This strongly suggests that peripheral blood monocytes differentiate into endothelial-like cells.
在含有4%人血清白蛋白的培养基中培养人脐静脉内皮细胞时，无需传代培养即可维持上皮样形态和功能数月。当将从外周血中收集并使其吞噬荧光乳胶珠的内皮细胞与标记的单核细胞/巨噬细胞（Mo/Phi）共培养时，一些Mo/Phi改变了形状，变成了与血管内皮细胞无法区分的上皮样细胞。这种转变在共培养的几个小时内就开始了。共培养开始7天后，超过一半的标记细胞在形态上被鉴定为内皮样细胞。此外，形态改变的Mo/Phi不表达Mo/Phi特异性抗原，即MHC II类分子和CD68，而是表达内皮细胞特异性抗原VE钙黏蛋白和vWF，并且转变为内皮样细胞的标记细胞不再吞噬荧光乳胶珠。这有力地表明外周血单核细胞可分化为内皮样细胞。
