Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, Witzenbichler B, Schatteman G, Isner J M
Department of Medicine (Cardiology), St. Elizabeth's Medical Center, Tufts University School of Medicine, 736 Cambridge Street, Boston, MA 02135, USA.
Science. 1997 Feb 14;275(5302):964-7. doi: 10.1126/science.275.5302.964.
Putative endothelial cell (EC) progenitors or angioblasts were isolated from human peripheral blood by magnetic bead selection on the basis of cell surface antigen expression. In vitro, these cells differentiated into ECs. In animal models of ischemia, heterologous, homologous, and autologous EC progenitors incorporated into sites of active angiogenesis. These findings suggest that EC progenitors may be useful for augmenting collateral vessel growth to ischemic tissues (therapeutic angiogenesis) and for delivering anti- or pro-angiogenic agents, respectively, to sites of pathologic or utilitarian angiogenesis.
通过基于细胞表面抗原表达的磁珠分选从人外周血中分离出假定的内皮细胞（EC）祖细胞或成血管细胞。在体外，这些细胞分化为内皮细胞。在缺血动物模型中，异种、同种和自体EC祖细胞整合到活跃血管生成部位。这些发现表明，EC祖细胞可能分别有助于增加缺血组织的侧支血管生长（治疗性血管生成）以及将抗血管生成或促血管生成剂递送至病理性或实用性血管生成部位。
