Kravchenko L P, Ludoviĭ V I, Bilous A M
Ukr Biokhim Zh. 1976 Jan-Feb;48(1):11-5.
The injury degree of lysosomes isolated from the rat liver under low temperature conditions was studied with respect to nonsedimented hydrolases activities (acid RNases, DNase, phosphatase, cathepsins) taken as a biochemical criterium of lysosomal membrane stability. The lysosomal fraction was resuspended in 0.15 M NaCl solution and frozen in the liquid N2 vapours up to --30 degrees C. According to the thermographic analysis data the samples were thawed and studied at different stages of cooling. The activity of the studied hydrolases was established to increase most considerably during the water crystallization period and at the NaCl eutectic point. Hydrolytic enzymes release from lysosomes proved to be dependent on the thawing rate and NaCl concentration. The obtained data indicate to the importance of concentration factors in lysosomal membrane cryoinjury.
以非沉降水解酶活性（酸性核糖核酸酶、脱氧核糖核酸酶、磷酸酶、组织蛋白酶）作为溶酶体膜稳定性的生化标准，研究了低温条件下从大鼠肝脏分离的溶酶体的损伤程度。将溶酶体部分重悬于0.15 M NaCl溶液中，并在液氮蒸气中冷冻至-30℃。根据热成像分析数据，将样品解冻并在冷却的不同阶段进行研究。结果表明，在所研究的水解酶中，其活性在水结晶期和NaCl共晶点时增加最为显著。溶酶体中水解酶的释放被证明取决于解冻速率和NaCl浓度。所得数据表明浓度因素在溶酶体膜冷冻损伤中的重要性。
