Bortnowski H B, Rosenthal R C
Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706.
Am J Vet Res. 1991 Oct;52(10):1636-8.
The role of L-asparaginase (L-ASP) in limiting signs of methotrexate (MTX) toxicosis was studied. Eight dogs were randomly allotted to 2 groups of 4 dogs. All dogs were given 400 IU of L-ASP/kg of body weight IM, on day 1. On day 10, group-1 dogs were given 3 mg of MTX/kg, IV, and group-2 dogs were given 6 mg of MTX/kg, IV. All dogs were given 400 IU of L-ASP/kg, IM, 24 hours later (on day 11). One group-2 dog was euthanatized on day 16 because of severe gastrointestinal signs that were unresponsive to treatment. A second dose of MTX, identical to that given on day 10, was given on day 20 to each surviving dog, followed by L-ASP on day 21. On day 67, the 7 surviving dogs were given 3 mg of MTX/kg, IV. Adverse reactions observed were vomiting, diarrhea, and weight loss. Gastrointestinal side effects of MTX were not attenuated with L-ASP and would be a serious limitation to use of MTX administered at an intermediate dose in the treatment of lymphoma in dogs.
研究了L-天冬酰胺酶（L-ASP）在减轻甲氨蝶呤（MTX）中毒症状方面的作用。八只狗被随机分为两组，每组四只。所有狗在第1天均接受400 IU/kg体重的L-ASP肌肉注射。在第10天，第1组狗静脉注射3 mg/kg的MTX，第2组狗静脉注射6 mg/kg的MTX。24小时后（第11天），所有狗均接受400 IU/kg的L-ASP肌肉注射。第2组的一只狗在第16天因严重的胃肠道症状且对治疗无反应而被安乐死。在第20天，给每只存活的狗注射与第10天相同剂量的MTX，随后在第21天注射L-ASP。在第67天，给7只存活的狗静脉注射3 mg/kg的MTX。观察到的不良反应有呕吐、腹泻和体重减轻。MTX的胃肠道副作用并未因L-ASP而减轻，这将严重限制在犬淋巴瘤治疗中使用中等剂量的MTX。
