免疫反应在金黄色葡萄球菌滤液制剂抗肿瘤作用中的参与情况。
Involvement of immune response in anti-tumor effects of Staphylococcus aureus filtrate preparation.
作者信息
Zhang Bo, Fang Yanfen, Cao Ji, Zhang Chong, Ying Meidan, He Qiaojun, Yang Bo
机构信息
Institute of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
出版信息
J Cancer Res Clin Oncol. 2008 Mar;134(3):373-9. doi: 10.1007/s00432-007-0293-y. Epub 2007 Aug 7.
PURPOSE
Staphylococcus Aureus Filtrate Preparation (SAFP) is a preparation of low-virulence strain of Staphylococcus aureus. In our study, we aimed to determine the anti-tumor activity of SAFP in vivo and explore the potential mechanism.
METHODS
Our study evaluated the anti-tumor activity of SAFP in four cancer cell models in vivo and determined its up-regulatory effects on mice immune system in vivo.
RESULTS
It showed that 7.2 ng/10 g SAFP could inhibit the progression of murine hepatoma H22 and sarcoma S180, and the tumor inhibition rates achieved 48.1 and 35.7%, respectively. In human non-small cell lung cancer A549 and hepatoma BEL7402 xenograft athymic mice models, T/C of 7.2 ng/10 g SAFP group reached 41.0% in A549 model and 21.0% in BEL7402 model. Meanwhile, SAFP also significantly increased the proliferation of cultured mice splenocytes to 162.1%, and 7.2 ng/kg SAFP significantly (P < 0.001) raised the activity of NK cells, elevated the serum level of IL-2, TNF-alpha and IFN-gamma in mice.
CONCLUSION
SAFP exhibited high efficiency in inhibiting the growth of cancer cells in a dose-dependent manner, which possibly attributed to its regulatory effects of immune system.
目的
金黄色葡萄球菌滤液制剂（SAFP）是一种低毒力金黄色葡萄球菌菌株的制剂。在我们的研究中，旨在确定SAFP在体内的抗肿瘤活性并探索其潜在机制。
方法
我们的研究评估了SAFP在四种体内癌细胞模型中的抗肿瘤活性，并确定了其对小鼠免疫系统的上调作用。
结果
结果显示，7.2 ng/10 g的SAFP可抑制小鼠肝癌H22和肉瘤S180的进展，肿瘤抑制率分别达到48.1%和35.7%。在人非小细胞肺癌A549和肝癌BEL7402异种移植裸鼠模型中，7.2 ng/10 g的SAFP组在A549模型中的T/C达到41.0%，在BEL7402模型中达到21.0%。同时，SAFP还显著将培养的小鼠脾细胞增殖提高到162.1%，7.2 ng/kg的SAFP显著（P < 0.001）提高了NK细胞的活性，提高了小鼠血清中IL-2、TNF-α和IFN-γ的水平。
结论
SAFP在抑制癌细胞生长方面表现出高效性，且呈剂量依赖性，这可能归因于其对免疫系统的调节作用。
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