Verschueren C P, Rutteman G R, Kuipers-Dijkshoorn N J, Sjollema B E, Vos J H, van Dijk J E, Cornelisse C J
Department of Clinical Sciences of Companion Animals, University of Utrecht, The Netherlands.
Anticancer Res. 1991 Sep-Oct;11(5):1755-61.
DNA ploidy was measured by flow cytometry in 36 primary malignant thyroid neoplasms (including 6 bilateral tumours which were considered as separate neoplasms) from 30 dogs. In addition, DNA ploidy was determined in local recurrences in 3 dogs, and in 18 metastatic sites from 14 dogs. Aneuploidy was found in 21 of 36 (58%) primary sites. Eighteen of the 21 (86%) aneuploid tumours contained hypodiploid cell populations, with 12 having single hypodiploid peaks, and 6 being multiploid. Three other tumours had single aneuploid peaks with a DNA index (DI) greater than 1.0. The DIs in local recurrences were identical to those in the original neoplasms. Ploidy status (diploid vs. aneuploid) was identical in primary and metastatic sites in 10 out of the 14 dogs. Aneuploidy was more frequent in carcinomas from dogs with distant metastases (78%) than from dogs with less advanced stages of disease (53%), although this difference was not significant. There was no significant correlation between DNA ploidy and histopathological variables. From the strikingly high frequency of hypodiploidy in canine tumours, it is concluded that ploidy evolution in canine neoplasms may differ from that in human tumours.
通过流式细胞术对30只犬的36个原发性甲状腺恶性肿瘤（包括6个双侧肿瘤，每个双侧肿瘤被视为独立的肿瘤）进行DNA倍性检测。此外，还对3只犬的局部复发肿瘤以及14只犬的18个转移部位进行了DNA倍性检测。在36个原发性肿瘤部位中，有21个（58%）检测到非整倍体。21个非整倍体肿瘤中有18个（86%）含有亚二倍体细胞群，其中12个有单个亚二倍体峰，6个为多倍体。另外3个肿瘤有单个非整倍体峰，DNA指数（DI）大于1.0。局部复发肿瘤的DI与原发肿瘤相同。14只犬中有10只犬的原发性和转移部位的倍性状态（二倍体与非整倍体）相同。远处转移犬的癌中非整倍体出现频率（78%）高于疾病分期较低犬的癌（53%），尽管这种差异不显著。DNA倍性与组织病理学变量之间无显著相关性。鉴于犬肿瘤中亚二倍体的出现频率极高，得出结论：犬肿瘤的倍性演变可能与人类肿瘤不同。
