Sangster Tim P, Wingate Julie E, Burton Lyle, Teichert Friederike, Wilson Ian D
Huntingdon Life Science, East Millstone, NJ 08875-2360, USA.
Rapid Commun Mass Spectrom. 2007;21(18):2965-70. doi: 10.1002/rcm.3164.
Sources of analytical variation in high-performance liquid chromatography/mass spectrometry (HPLC/MS), such as changes in retention, mass accuracy or signal intensity, have been investigated to assess their importance as a variable in the metabonomic analysis of human urine. In this study chromatographic retention and mass accuracy were found to be quite reproducible with the most significant source of analytical variation in the data sets obtained being the result of changes in detector response. Depending on the signal intensity threshold used to define the presence of a peak a sample component could be present in some replicate injections and absent in others within the same run. The implementation of a more sophisticated data software analysis package was found to greatly reduce the impact of detector response variability resulting in improved data analysis.
高效液相色谱/质谱联用（HPLC/MS）分析中的变异来源，如保留时间变化、质量准确度或信号强度，已被研究以评估其在人体尿液代谢组学分析中作为变量的重要性。在本研究中，发现色谱保留和质量准确度具有相当高的重现性，所获得数据集中分析变异的最主要来源是检测器响应的变化。根据用于定义峰存在的信号强度阈值，样品组分在同一次运行的某些重复进样中可能存在，而在其他进样中可能不存在。结果发现，实施更复杂的数据软件分析包可大大降低检测器响应变异性的影响，从而改善数据分析。
