Zhang Fang, Hang Xiaomin, Fan Xiaobing, Li Guijie, Yang Hong
School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, PR China.
Anaerobe. 2007 Oct-Dec;13(5-6):185-92. doi: 10.1016/j.anaerobe.2007.06.001. Epub 2007 Jun 13.
A selection and optimization procedure for the synbiotic combination of probiotic and prebiotics was established to optimize its cholesterol removal in vitro. In light of fermentability, prebiotics utilization by probiotics was highly variable and interspecies differences existed. Based on the results of fermentability, L. plantarum LS12, Ls31, LP529 and L. ruminis La3 could be the better candidates for symbiotic research. The bile tolerance of all the tested strains could be improved by the strain-specific prebiotics comparing to the control carbon source (glucose). The strain LS12 was finally selected to form the symbiotic according to its better ability to ferment prebiotics and bile tolerance, while the five prebiotics (FOS, stachyose, GOS, IMO and mannitol) were selected to make their synbiotic combination because of their better enhancement of bile tolerance and growth support to LS12. The synbiotic combination for cholesterol removal was optimized by use of response surface methodology. The first-order model showed that the selected prebiotics mannitol and GOS were significant factors. Then through the second-order polynomial regression model, the optimum conditions of the two factors for cholesterol removal by the synbiotic were suggested.
建立了益生菌和益生元合生元组合的筛选与优化程序，以优化其体外胆固醇去除效果。鉴于发酵能力，益生菌对益生元的利用差异很大，且存在种间差异。基于发酵能力的结果，植物乳杆菌LS12、Ls31、LP529和瘤胃乳杆菌La3可能是共生研究的较好候选菌株。与对照碳源（葡萄糖）相比，菌株特异性益生元可提高所有测试菌株的胆汁耐受性。最终选择菌株LS12形成共生体，因为其具有更好的益生元发酵能力和胆汁耐受性，同时选择了五种益生元（低聚果糖、水苏糖、低聚半乳糖、异麦芽酮糖醇和甘露醇）来制备它们的合生元组合，因为它们对LS12的胆汁耐受性增强和生长支持效果更好。利用响应面法对用于胆固醇去除的合生元组合进行了优化。一阶模型表明，所选的益生元甘露醇和低聚半乳糖是显著因素。然后通过二阶多项式回归模型，提出了合生元去除胆固醇的两个因素的最佳条件。
