多唾液酸化CD56对自然杀伤细胞细胞毒性的影响。
Impact of polysialylated CD56 on natural killer cell cytotoxicity.
作者信息
Moebius Jeannette M, Widera Darius, Schmitz Juergen, Kaltschmidt Christian, Piechaczek Christoph
机构信息
Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
出版信息
BMC Immunol. 2007 Aug 6;8:13. doi: 10.1186/1471-2172-8-13.
Abstract
BACKGROUND
Siglec-7, a sialic acid binding inhibitory receptor expressed by NK cells is masked in vivo by a so far unknown ligand. It shows a strong binding prevalence for alpha-2,8-linked disialic acids in vitro.
RESULTS
Here we describe the expression of PSA-NCAM (alpha-2,8-linked polysialic acid modified NCAM) on functional adult peripheral blood natural killer cells and examine its possible role in masking Siglec-7. Unmasking of Siglec-7 using Clostridium perfringens neuraminidase massively reduces NK cell cytotoxicity. By contrast a specific removal of PSA using Endo-NF does not lead to a reduction of NK cell cytotoxicity.
CONCLUSION
The results presented here therefore indicate that PSA-NCAM is not involved in masking Siglec-7.
摘要
背景
唾液酸结合免疫球蛋白样凝集素-7（Siglec-7）是一种由自然杀伤（NK）细胞表达的唾液酸结合抑制性受体，在体内被一种迄今未知的配体所掩盖。它在体外对α-2,8-连接的二唾液酸具有很强的结合优势。
结果
在此我们描述了多唾液酸神经细胞黏附分子（PSA-NCAM，α-2,8-连接的多唾液酸修饰的神经细胞黏附分子）在功能性成人外周血自然杀伤细胞上的表达，并研究其在掩盖Siglec-7方面的可能作用。使用产气荚膜梭菌神经氨酸酶解开Siglec-7会大幅降低NK细胞的细胞毒性。相比之下，使用内切神经氨酸酶NF特异性去除PSA并不会导致NK细胞细胞毒性降低。
结论
因此，此处给出的结果表明PSA-NCAM不参与掩盖Siglec-7。
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