人蜕膜自然杀伤细胞是具有免疫调节潜能的独特自然杀伤细胞亚群。

Human decidual natural killer cells are a unique NK cell subset with immunomodulatory potential.

作者信息

Koopman Louise A, Kopcow Hernan D, Rybalov Basya, Boyson Jonathan E, Orange Jordan S, Schatz Frederick, Masch Rachel, Lockwood Charles J, Schachter Asher D, Park Peter J, Strominger Jack L

机构信息

Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.

出版信息

J Exp Med. 2003 Oct 20;198(8):1201-12. doi: 10.1084/jem.20030305.

Natural killer cells constitute 50-90% of lymphocytes in human uterine decidua in early pregnancy. Here, CD56(bright) uterine decidual NK (dNK) cells were compared with the CD56(bright) and CD56(dim) peripheral NK cell subsets by microarray analysis, with verification of results by flow cytometry and RT-PCR. Among the approximately 10,000 genes studied, 278 genes showed at least a threefold change with P < or = 0.001 when comparing the dNK and peripheral NK cell subsets, most displaying increased expression in dNK cells. The largest number of these encoded surface proteins, including the unusual lectinlike receptors NKG2E and Ly-49L, several killer cell Ig-like receptors, the integrin subunits alpha(D), alpha(X), beta1, and beta5, and multiple tetraspanins (CD9, CD151, CD53, CD63, and TSPAN-5). Additionally, two secreted proteins, galectin-1 and progestagen-associated protein 14, known to have immunomodulatory functions, were selectively expressed in dNK cells.

在妊娠早期，自然杀伤细胞占人子宫蜕膜中淋巴细胞的50 - 90%。在此，通过微阵列分析将CD56（明亮型）子宫蜕膜自然杀伤（dNK）细胞与CD56（明亮型）和CD56（暗淡型）外周自然杀伤细胞亚群进行比较，并通过流式细胞术和逆转录-聚合酶链反应验证结果。在研究的约10,000个基因中，当比较dNK细胞和外周自然杀伤细胞亚群时，278个基因显示至少有三倍的变化且P≤0.001，大多数在dNK细胞中表达增加。其中编码表面蛋白的数量最多，包括不寻常的凝集素样受体NKG2E和Ly - 49L、几种杀伤细胞免疫球蛋白样受体、整合素亚基α(D)、α(X)、β1和β5以及多种四跨膜蛋白（CD9、CD151、CD53、CD63和TSPAN - 5）。此外，已知具有免疫调节功能的两种分泌蛋白，即半乳糖凝集素-1和孕激素相关蛋白14，在dNK细胞中选择性表达。