Törmä H, Vahlquist A
Department of Dermatology, University Hospital, Linköping, Sweden.
Skin Pharmacol. 1991;4(3):154-7. doi: 10.1159/000210942.
The concentration of 3,4-didehydroretinol, an epidermal metabolite of retinol, is changed both in association with keratinizing disorders and during treatment with certain synthetic retinoids. To delineate further the factors regulation biosynthesis of 3,4-didehydroretinol, keratinocytes from three different layers of normal epidermis were prepared by trypsinization and incubated with [3H]-retinol for 20 h. Cell-bound [3H]-retinol and 3,4-[3H]-didehydroretinol were separated by high-performance liquid chromatography. The results indicate that the uptake of retinol and the synthesis of 3,4-didehydroretinol occur predominantly in undifferentiated cells from the basal and spinous layers of the epidermis.
视黄醇的表皮代谢产物3,4-二脱氢视黄醇的浓度在与角化性疾病相关时以及在使用某些合成视黄酸治疗期间都会发生变化。为了进一步阐明调节3,4-二脱氢视黄醇生物合成的因素，通过胰蛋白酶消化制备了来自正常表皮三个不同层的角质形成细胞，并与[3H]-视黄醇一起孵育20小时。通过高效液相色谱法分离细胞结合的[3H]-视黄醇和3,4-[3H]-二脱氢视黄醇。结果表明，视黄醇的摄取和3,4-二脱氢视黄醇的合成主要发生在表皮基底层和棘层的未分化细胞中。
