Nyangao J W O, Kingori P, Okoth F A
Centre for Virus Research, Kenya Medical Research Institute, P.O. Box 54628-00200, Nairobi, Kenya.
East Afr Med J. 2006 Dec;83(12):666-9.
Gastro-enteritis continues to be a significant cause of mortality in infants and young children in developing countries. Some previous studies have associated echoviruses with gastroenteritis.
To look for other viral agents causing gastro-enteritis in stool samples of infants and young children admitted with diarrhoea in an urban hospital in Kenya by electron microscopy.
A cross sectional study.
Mbagathi District Hospital, Nairobi, Kenya.
One hundred infants and young children admitted in the hospital due to gastro-enteritis and found to be negative for group A rotaviruses.
We report a virus designated KE/CVR-99 which was recovered from the faeces of a one year old male child hospitalised for gastro-enteritis. Virus particles measuring 20-25 nm in diameter were detected by electron microscopy from the stool of the child hospitalised with gastro-enteritis. After injecting the viral particles intracerebrally in newborn suckling mice which were then observed daily for signs of illness, no sign of paralysis or any other form of illness was detected. Cytopathic effect was observed in rhabdomyosarcoma cells within six days of incubation whereas no cytopathic effect was observed in L-20 B cells. We identified the virus after typing against known anti-sera to a panel of enteroviruses. This virus was found to be Echovirus 7.
This is the first case report of echovirus 7 in association with gastro-enteritis and detection by electron microscopy in Kenya.
在发展中国家，肠胃炎仍是婴幼儿死亡的一个重要原因。此前一些研究已将埃可病毒与肠胃炎联系起来。
通过电子显微镜检查，在肯尼亚一家城市医院收治的腹泻婴幼儿粪便样本中寻找导致肠胃炎的其他病毒病原体。
一项横断面研究。
肯尼亚内罗毕的姆巴加蒂区医院。
100名因肠胃炎入院且A组轮状病毒检测呈阴性的婴幼儿。
我们报告了一种名为KE/CVR - 99的病毒，它从一名因肠胃炎住院的1岁男童粪便中分离出来。通过电子显微镜从该肠胃炎住院患儿的粪便中检测到直径为20 - 25纳米的病毒颗粒。将病毒颗粒脑内注射给新生乳鼠后，每天观察其发病迹象，未检测到麻痹迹象或任何其他形式的疾病。横纹肌肉瘤细胞在培养6天内出现细胞病变效应，而L - 20 B细胞未出现细胞病变效应。我们用一组肠道病毒的已知抗血清进行分型后鉴定出该病毒。发现这种病毒是埃可病毒7型。
这是肯尼亚首例关于埃可病毒7型与肠胃炎相关并通过电子显微镜检测到的病例报告。
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