Pupilli C, Gomez R A, Tuttle J B, Peach M J, Carey R M
Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville 22908.
Pediatr Nephrol. 1991 Nov;5(6):690-5. doi: 10.1007/BF00857873.
The development of renin-containing cells and nerve fibers was studied in Sprague-Dawley rat kidneys during the last third of gestation and the first 15 days of postnatal life. Kidney tissue sections were stained for nerve fibers or double stained employing an anti-rat renin polyclonal antibody and a monoclonal antibody (TUJ1) directed against a neuron-specific class III beta-tubulin isotype. Renin-containing cells and nerve fibers were detected at 17 days of gestation, in close spatial relationship along the main branches of the renal artery. During fetal life, renin-containing cells and nerve fibers were spatially associated along arcuate and interlobular arteries, renin-containing cells being also present throughout the entire length of afferent arterioles supplying juxtamedullary glomeruli. During postnatal life the distribution of renin-containing cells progressively shifted to a restricted juxtaglomerular position in afferent arterioles. Simultaneously, density and organization of nerve fibers increased with age along the arterial vascular tree. Our results suggest that innervation of renin-containing cells is present in fetal life and follows the centrifugal pattern of renin distribution and nephrovascular development.
在妊娠后期三分之一阶段以及出生后15天内，对斯普拉格-道利大鼠肾脏中含肾素细胞和神经纤维的发育情况进行了研究。肾脏组织切片进行神经纤维染色，或使用抗大鼠肾素多克隆抗体和针对神经元特异性III类β-微管蛋白同种型的单克隆抗体（TUJ1）进行双重染色。在妊娠17天时检测到含肾素细胞和神经纤维，它们沿着肾动脉的主要分支在空间上紧密相关。在胎儿期，含肾素细胞和神经纤维在弓形动脉和小叶间动脉周围在空间上相关联，含肾素细胞也存在于供应近髓肾单位的入球小动脉的整个长度上。在出生后，含肾素细胞的分布逐渐转移到入球小动脉中局限的球旁位置。同时，随着年龄增长，神经纤维的密度和组织结构沿着动脉血管树增加。我们的结果表明，含肾素细胞的神经支配在胎儿期就已存在，并遵循肾素分布和肾血管发育的离心模式。
