[氯化钾协同转运蛋白1表达在宫颈癌组织中的意义]

[The significance of K-Cl cotransport1 expression in cervical cancer tissues].

作者信息

Lu Xiao-yun, Zhang Shu-lan, Lu Yan-ming, Xiu Xiao-xin, Sun Xiao-wei

机构信息

Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang 110004, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2007 May 8;87(17):1156-9.

PMID:17686231

Abstract

OBJECTIVE

To investigate the expression of K-Cl cotransport 1(KCC1) in cervical cancer tissues and to investigate its role in the onset and progression of cervical cancer.

METHODS

Specimens of uterus were obtained from 60 patients aged 45.89 (25 approximately 73), 40 with cervical cancer, 10 with cervical intraepithelial neoplasia (CIN), and 10 with chronic cervicitis. Semi-quantitative RT-PCR was used to detect the mRNA expression. Western blotting was used to detect the protein expression of KCC1. Immunofluorescence assay was used to detect the location of KCC1 protein.

RESULTS

The mRNA expression and protein expression of KCC1 were both significantly higher in the cervical cancer tissue than those in the CIN and chronic cervicitis tissues (all P < 0.05). The levels of KCC1 in the lowly differentiated cancer tissues (at grades G(2) and G(3)) were significantly higher than those in the highly differentiated cancer tissues (at grade G(1), P < 0.05). Western blotting revealed that the protein expression level of KCC1 was significantly higher in the cervical cancer tissues than in the CIN and chronic cervicitis tissues (both P < 0.05) and the protein expression levels of KCC1 in the cancer tissues at G" 2 and G(3) grades were significantly higher than that in the cancer tissues at grade G(1) (both P < 0.05). There were no significant differences in mRNA and protein expression between the early and terminal cervical cancer tissues. There were no significant differences in the mRNA and protein expression of KCC1 between the cervical cancer cases with or without lymph node metastasis. Immunofluorescence assay showed that KCC1 was located in the cellular membrane in all patients and the KCC1 expression level there was significantly higher in the cervical cancer tissues than in the tissues of CIN and chronic cervicitis.

CONCLUSION

The expression of KCC1 is up-regulated in cervical cancer and it may play an important role in the onset and progression of cervical cancer.

摘要

目的

研究钾氯共转运体1（KCC1）在宫颈癌组织中的表达情况，并探讨其在宫颈癌发生发展中的作用。

方法

收集60例患者的子宫标本，患者年龄45.89岁（25至73岁），其中40例为宫颈癌患者，10例为宫颈上皮内瘤变（CIN）患者，10例为慢性宫颈炎患者。采用半定量逆转录聚合酶链反应（RT-PCR）检测KCC1的mRNA表达。采用蛋白质印迹法检测KCC1的蛋白表达。采用免疫荧光法检测KCC1蛋白的定位。

结果

宫颈癌组织中KCC1的mRNA表达和蛋白表达均显著高于CIN组织和慢性宫颈炎组织（均P<0.05）。低分化癌组织（G2和G3级）中KCC1的水平显著高于高分化癌组织（G1级，P<0.05）。蛋白质印迹法显示，宫颈癌组织中KCC1的蛋白表达水平显著高于CIN组织和慢性宫颈炎组织（均P<0.05），G2和G3级癌组织中KCC1的蛋白表达水平显著高于G1级癌组织（均P<0.05）。早期和晚期宫颈癌组织之间的mRNA和蛋白表达无显著差异。有或无淋巴结转移的宫颈癌病例之间KCC1的mRNA和蛋白表达无显著差异。免疫荧光法显示，所有患者的KCC1均位于细胞膜上，宫颈癌组织中KCC1的表达水平显著高于CIN组织和慢性宫颈炎组织。