Muralidharan Bhavana, Bakthavachalu Baskar, Pathak Anuj, Seshadri Vasudevan
National Centre for Cell Science, Ganeshkhind, Pune 411007, India.
FEBS Lett. 2007 Aug 21;581(21):4103-8. doi: 10.1016/j.febslet.2007.07.050. Epub 2007 Jul 31.
Glucose induced translation of insulin in pancreatic beta cells is mediated by the 5'UTR of insulin mRNA. We determined the minimal sequence/structure in the 5'UTR of rat insulin gene1 for this regulation. We show that specific factors in the pancreatic islets bind to the 5'UTR of the insulin mRNA upon glucose stimulation. We identified a minimal 29-nucleotide element in the 5'UTR that is sufficient to form the complex, and confer glucose mediated translation activation. Conserved residues in the predicted stem loop region of the un-translated region (UTR) seem to be important for the complex formation and the translation regulation.
葡萄糖诱导胰腺β细胞中胰岛素的翻译是由胰岛素mRNA的5'非翻译区介导的。我们确定了大鼠胰岛素基因1的5'非翻译区中参与该调节的最小序列/结构。我们发现，葡萄糖刺激后，胰岛中的特定因子会与胰岛素mRNA的5'非翻译区结合。我们在5'非翻译区鉴定出一个最小的29个核苷酸的元件，该元件足以形成复合物，并赋予葡萄糖介导的翻译激活作用。非翻译区（UTR）预测的茎环区域中的保守残基似乎对复合物的形成和翻译调节很重要。
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