Torsteinsdóttir Sigurbjörg, Carlsdóttir Helga María, Svansson Vilhjálmur, Matthíasdóttir Sigrídur, Martin Agnes Helga, Pétursson Gudmundur
Institute for Experimental Pathology, University of Iceland, Keldur v/Vesturlandsveg, IS-112 Reykjavík, Iceland.
Vaccine. 2007 Sep 17;25(37-38):6713-20. doi: 10.1016/j.vaccine.2007.07.004. Epub 2007 Jul 26.
In spite of intense efforts no vaccine is yet available that protects against lentiviral infections. Sheep were immunised eight times over a period of 2.5 years with the maedi-visna (MVV) gag gene on two different vectors, 2 sheep with VR1012-gag-CTE and 2 sheep with pcDNA3.1-gag-CTE. All sheep responded to some of the mature MVV Gag proteins in Western blot (WB). Three of them responded to the virus in lymphocyte proliferation test. The sheep received a boost with recombinant Gag protein resulting in elevated antibody response. However, when they were challenged intratracheally with MVV they all became immediately infected as judged by a strong rise in antibody titer and virus isolation from blood. It is therefore clear that the vaccination gave no protection. It is even possible that it facilitated infectivity since virus was isolated earlier from all the vaccinated sheep than from any of the unvaccinated sheep infected in the same way with the same dose.
尽管付出了巨大努力，但目前仍没有可预防慢病毒感染的疫苗。在2.5年的时间里，用两种不同载体上的梅迪-维斯纳病毒（MVV）gag基因对绵羊进行了8次免疫，2只绵羊用VR1012-gag-CTE免疫，2只绵羊用pcDNA3.1-gag-CTE免疫。所有绵羊在蛋白质免疫印迹（WB）中均对一些成熟的MVV Gag蛋白产生反应。其中3只在淋巴细胞增殖试验中对病毒产生反应。这些绵羊接受了重组Gag蛋白的加强免疫，导致抗体反应升高。然而，当它们经气管内接种MVV时，根据抗体滴度的大幅上升和从血液中分离出病毒判断，它们都立即被感染。因此，很明显疫苗没有提供保护作用。甚至有可能疫苗促进了感染性，因为从所有接种疫苗的绵羊中比从以相同方式、相同剂量感染的任何未接种疫苗的绵羊中更早地分离出了病毒。
