Fridman O, Foglia V G, De Nicola A F
Acta Physiol Lat Am. 1975;25(1):52-9.
Short and prolonged administration of ACTH was used to study the effects of the hormone on protein synthesis in two subcellular fractions obtained from rat adrenals incubated in the presence of a (14C)-labelled amino acid mixture. Acute treatment with ACTH produced an increased labelling of the proteins of the post-mitochondrial (POST-MIT) fraction only. On the other hand, treatment with ACTH for 4-28 days, significantly increased the specific activity of proteins in the mitochondrial (MIT) fraction, without change in the radiolabelling of the POST-MIT fraction. The incorporation of (14C)-labelled amino acids into the proteins of the MIT and POST-MIT fractions, from control or ACTH-treated adrenals, was reduced by inhibitors of cytoplasmic protein synthesis (puromycin and cycloheximide). This suggests that the increased labelling of the MIT fraction after prolonged ACTH might be related to a translocation of cytoplasmic proteins to the mitochondrion.
使用促肾上腺皮质激素（ACTH）的短期和长期给药来研究该激素对从在含有（14C）标记氨基酸混合物的条件下孵育的大鼠肾上腺获得的两个亚细胞组分中蛋白质合成的影响。ACTH的急性处理仅使线粒体后（POST-MIT）组分的蛋白质标记增加。另一方面，ACTH处理4-28天显著增加了线粒体（MIT）组分中蛋白质的比活性，而POST-MIT组分的放射性标记没有变化。细胞质蛋白质合成抑制剂（嘌呤霉素和环己酰亚胺）降低了来自对照或ACTH处理肾上腺的（14C）标记氨基酸掺入MIT和POST-MIT组分蛋白质中的量。这表明长期ACTH处理后MIT组分标记增加可能与细胞质蛋白质向线粒体的转运有关。
