Nahori M A, Prevost M C, Gounon P
Unité de Pharmacologie Cellulaire, Unité Associée Institut Pasteur INSERM 285, Paris, France.
J Lipid Mediat. 1991 Jan-Feb;3(1):101-12.
The tracheo-bronchial epithelial cells are the first cells of the respiratory tract to encounter inhaled pathogens or allergens. In order to study these interactions in vitro we developed a culture of epithelial cells from guinea pig trachea which were kept as a primary culture for 3-13 days. Electron microscopy showed that the specific ultrastructure of epithelial cells was maintained, particularly cytokeratin intermediate filaments detected by immunofluorescence. The cells in culture produced eicosanoid metabolites, mainly leukotriene B4 and prostaglandin E2 spontaneously and upon calcium ionophore A23187 stimulation.
气管支气管上皮细胞是呼吸道中最先接触吸入病原体或过敏原的细胞。为了在体外研究这些相互作用，我们培养了豚鼠气管的上皮细胞，并将其作为原代培养物保存3 - 13天。电子显微镜显示上皮细胞的特定超微结构得以维持，尤其是通过免疫荧光检测到的细胞角蛋白中间丝。培养的细胞自发地以及在钙离子载体A23187刺激下产生类花生酸代谢产物，主要是白三烯B4和前列腺素E2。
